产品: HDAC4 抗体
货号: AF6349
描述: Rabbit polyclonal antibody to HDAC4
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
分子量: 119kDa; 119kD(Calculated).
蛋白号: P56524
RRID: AB_2835043

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HDAC4 Antibody detects endogenous levels of total HDAC4.
RRID:
AB_2835043
引用格式: Affinity Biosciences Cat# AF6349, RRID:AB_2835043.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AHO3; BDMR; EC 3.5.1.98; HA6116; HD 4; HD4; HDAC 4; HDAC A; HDAC4; HDAC4_HUMAN; HDACA; Histone deacetylase 4; Histone Deacetylase A; KIAA0288;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P56524 HDAC4_HUMAN:

Ubiquitous.

描述:
HDAC4 a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4.
序列:
MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFPVSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P56524 作为底物

Site PTM Type Enzyme
T36 Phosphorylation
S45 Phosphorylation
S58 Phosphorylation
K79 Ubiquitination
K151 Ubiquitination
S168 Phosphorylation
S196 Phosphorylation
S210 Phosphorylation Q13557 (CAMK2D)
S215 Phosphorylation
Y225 Phosphorylation
Y233 Phosphorylation
K236 Acetylation
K243 Ubiquitination
S246 Phosphorylation Q7KZI7 (MARK2) , P57059 (SIK1) , O43318 (MAP3K7)
S265 Phosphorylation Q96GD4 (AURKB)
S266 Phosphorylation
T278 Phosphorylation
S298 Phosphorylation P49841 (GSK3B)
S302 Phosphorylation P49841 (GSK3B)
S339 Phosphorylation
S348 Phosphorylation
S350 Phosphorylation
T391 Phosphorylation
T394 Phosphorylation
S400 Phosphorylation
T465 Phosphorylation
S467 Phosphorylation P57059 (SIK1)
K494 Ubiquitination
Y540 Phosphorylation
K548 Acetylation
K559 Sumoylation
S565 Phosphorylation
S584 Phosphorylation
S611 Phosphorylation
S632 Phosphorylation Q13557 (CAMK2D)
S633 Phosphorylation
S636 Phosphorylation
T638 Phosphorylation
S642 Phosphorylation
T648 Phosphorylation
K705 Ubiquitination
K737 Ubiquitination
S741 Phosphorylation
S744 Phosphorylation
S1025 Phosphorylation
K1026 Ubiquitination
T1057 Phosphorylation
S1061 Phosphorylation

研究背景

功能:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1.

翻译修饰:

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.

Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

细胞定位:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation. Interaction with SIK3 leads to HDAC4 retention in the cytoplasm (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2A. Interacts with MEF2C and MEF2D. Interacts with AHRR (By similarity). Interacts with NR2C1. Interacts with HDAC7 (By similarity). Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B (By similarity). Interacts with KDM5B. Interacts with MYOCD (By similarity). Interacts with MORC2. Interacts (via PxLPxI/L motif) with ANKRA2 (via ankyrin repeats). Interacts with CUL7 (as part of the 3M complex); negatively regulated by ANKRA2. Interacts with EP300 in the presence of TFAP2C. Interacts with HSPA1A and HSPA1B leading to their deacetylation at 'Lys-77'. Interacts with ZBTB7B; the interaction allows the recruitment of HDAC4 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression (By similarity). Interacts with DHX36 (By similarity). Interacts with SIK3; this interaction leads to HDAC4 retention in the cytoplasm (By similarity). Interacts with ZNF638.

蛋白家族:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

The PxLPxI/L motif mediates interaction with ankyrin repeats of ANKRA2.

Belongs to the histone deacetylase family. HD type 2 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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