产品: WEE1 抗体
货号: AF6814
描述: Rabbit polyclonal antibody to WEE1
应用: WB
反应: Human, Mouse, Rat
分子量: 55kD(isoform), 72~100kD; 72kD(Calculated).
蛋白号: P30291
RRID: AB_2847537

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
WEE1 Antibody detects endogenous levels of total WEE1.
RRID:
AB_2847537
引用格式: Affinity Biosciences Cat# AF6814, RRID:AB_2847537.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686I18166; EC 2.7.10.2; FLJ16446; MGC105683; OTTHUMP00000231338; OTTHUMP00000231339; Wee 1; WEE 1 homolog 1 (S. pombe); WEE1; WEE1 homolog (S. pombe); Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1, S. pombe, homolog of; WEE1, somatic; Wee1-like protein kinase; WEE1_HUMAN; WEE1A; Wee1A kinase; WEE1hu;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

翻译修饰 - P30291 作为底物

Site PTM Type Enzyme
Phosphorylation
R14 Methylation
S48 Phosphorylation
T49 Phosphorylation
S53 Phosphorylation P53350 (PLK1)
S67 Phosphorylation
T69 Phosphorylation
S78 Phosphorylation
S85 Phosphorylation
S121 Phosphorylation P68400 (CSNK2A1)
S123 Phosphorylation P53350 (PLK1) , P06493 (CDK1) , P24941 (CDK2)
S127 Phosphorylation
Y132 Phosphorylation
S137 Phosphorylation
S139 Phosphorylation
S150 Phosphorylation
S165 Phosphorylation
T173 Phosphorylation
T187 Phosphorylation
T190 Phosphorylation
S193 Phosphorylation
S196 Phosphorylation
R199 Methylation
K207 Ubiquitination
S211 Phosphorylation P49841 (GSK3B)
S212 Phosphorylation P48730 (CSNK1D)
T217 Phosphorylation
T239 Phosphorylation
S242 Phosphorylation
Y258 Phosphorylation
S262 Phosphorylation
S270 Phosphorylation
Y272 Phosphorylation
K292 Ubiquitination
S293 Phosphorylation
K304 Ubiquitination
S307 Phosphorylation
S312 Phosphorylation
K315 Ubiquitination
S444 Phosphorylation
K460 Ubiquitination
S472 Phosphorylation
S480 Phosphorylation
K547 Ubiquitination
S571 Phosphorylation
S576 Phosphorylation
K588 Ubiquitination
K590 Sumoylation
K590 Ubiquitination
K596 Ubiquitination
K600 Ubiquitination
R617 Methylation
S642 Phosphorylation Q8IWQ3 (BRSK2) , Q8TDC3 (BRSK1) , P31749 (AKT1)
Y646 Phosphorylation

翻译修饰 - P30291 作为激酶

Substrate Site Source
P06493-2 (CDK1) Y15 Uniprot
P07900 (HSP90AA1) Y38 Uniprot
P24941 (CDK2) T14 Uniprot
P24941-1 (CDK2) Y15 Uniprot
P30291-1 (WEE1) T173 Uniprot

研究背景

功能:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

翻译修饰:

Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.

Dephosphorylated at Thr-239 by CTDP1.

Ubiquitinated and degraded at the onset of G2/M phase.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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