| 产品: | ICK 抗体 |
| 货号: | AF6816 |
| 描述: | Rabbit polyclonal antibody to ICK |
| 应用: | IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 71kD(Calculated). |
| 蛋白号: | Q9UPZ9 |
| RRID: | AB_2847539 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6816, RRID:AB_2847539.
展开/折叠
2210420N10Rik; AI848300; ECO; Heart serine threonine protein kinase; hICK; Intestinal cell (MAK like) kinase; Intestinal cell kinase; KIAA0936; Laryngeal cancer kinase 2; LCK2; MAK related kinase; MGC46090; mICK; MRK; OTTMUSP00000041856; OTTMUSP00000041857; Serine/threonine protein kinase ICK;
抗原和靶标
A synthesized peptide derived from human ICK.
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
- Q9UPZ9 CILK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALRYPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKPKSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYASRR
翻译修饰 - Q9UPZ9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | P50613 (CDK7) , Q8IZL9 (CDK20) | Uniprot |
| Y159 | Phosphorylation | Q9UPZ9 (ICK) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| K491 | Methylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| K562 | Acetylation | Uniprot | |
| Y583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot |
翻译修饰 - Q9UPZ9 作为激酶
研究背景
Required for ciliogenesis. Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).
Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity.
Nucleus. Cytoplasm>Cytosol. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Also found at the ciliary tip (PubMed:24797473). Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360, PubMed:19185282).
Cytoplasm.
Note: Predominant cytoplasmic localization has been observed with a N-terminally GFP-tagged construct.
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
限制条款
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