p57 Kip2 Antibody - #AF6819

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产品: p57 Kip2 抗体
货号: AF6819
描述: Rabbit polyclonal antibody to p57 Kip2
应用: WB
反应: Human
分子量: 32kD(Calculated).
蛋白号: P49918
RRID: AB_2847542

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
p57 Kip2 Antibody detects endogenous levels of total p57 Kip2.
RRID:
AB_2847542
引用格式: Affinity Biosciences Cat# AF6819, RRID:AB_2847542.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Beckwith Wiedemann syndrome; BWCR; BWS; CDKI; CDKN 1C; CDKN1C; CDN1C_HUMAN; Cyclin dependent kinase inhibitor 1C; Cyclin dependent kinase inhibitor p57; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; KIP 2; KIP2; p57; p57 Kip 2; p57KIP2; WBS;

抗原和靶标

免疫原:

A synthesized peptide derived from human p57 Kip2.

Uniprot:

P49918(CDN1C_HUMAN) >>Visit HPA database.

基因/基因ID:
CDKN1C(1028)
表达:
P49918 CDN1C_HUMAN:

Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

序列:
MSDASLRSTSTMERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTEVDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVPAPASTPPPVPVLAPAPAPAPAPVAAPVAAPVAVAVLAPAPAPAPAPAPAPAPVAAPAPAPAPAPAPAPAPAPAPDAAPQESAEQGANQGQRGQEPLADQLHSGISGRPAAGTAAASANGAAIKKLSGPLISDFFAKRKRSAPEKSSGDVPAPCPSPSAAPGVGSVEQTPRKRLR

翻译修饰 - P49918 作为底物

Site PTM Type Enzyme
S43 Phosphorylation
S146 Phosphorylation Q16539 (MAPK14)
K266 Acetylation
S268 Phosphorylation
K278 Acetylation
S282 Phosphorylation P31749 (AKT1)
S297 Phosphorylation
S299 Phosphorylation
S306 Phosphorylation
T310 Phosphorylation P31749 (AKT1)

研究背景

功能:

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

亚基结构:

Interacts with PCNA.

蛋白家族:

Belongs to the CDI family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

文献引用

1). GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer. OncoTargets and Therapy, 2020 (PubMed: 32922038) [IF=4.0]

Application: WB    Species: Human    Sample: gastric cancer cell

Figure 5 Overexpression of GCNT4 inhibits GC cell growth. (A) GCNT4 baseline mRNA and protein expression levels in 5 gastric cancer cell lines and one normal gastric epithelial cell line (GES-1) by RT-qPCR (upper panel) and Western blotting (down panel); (mean±SD, with one-way ANOVA). (B) The efficiency of GCNT4 overexpression were validated by qRT–PCR (upper panel) and Western blotting (down panel) in SGC-7901 and MGC-803 (mean±SD, with paired t-test); P<0.001. (C) CCK-8 assay showing GCNT4 overexpression inhibited SGC7901 and MGC-803 proliferation compared with control cells (mean±SD, with paired t-test); *P<0.01. (D) A flow cytometry assays showing GCNT4 overexpression arrested SGC-7901 and MGC-803 cell cycle at G2/M phase. (E) Western-blot analysis for the protein level of indicated molecules in GCNT4-overexpressing SGC-7901 and MGC-803 for 48 hours.
2). Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer. Oncology Letters, 2017 (PubMed: 29085511) [IF=2.9]

Application: WB    Species: human    Sample: A549 cells

Figure 4.| E2F3 promotes NSCLC proliferation through regulating the cell cycle in NSCLC. The expression of cell cycle‑associated proteins in A549 were detected by western blot (A). E2F3 can rescue the cell growth inhibition induced by decreased NEAT1 (B). P<0.05.
3). Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development. Oncotarget, 2016 (PubMed: 26840018)

Application: WB    Species:    Sample:

(G–H) Expression of cyclin D1, cyclin D1, CDK4, p21 and p57 protein in transfected A549 and SPC-A-1 cells. Assays were performed in triplicate. Means ± SEM was shown. Statistical analysis was conducted using student t-test.

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