| 产品: | AOS1 抗体 |
| 货号: | AF0266 |
| 描述: | Rabbit polyclonal antibody to AOS1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 38kDa; 38kD(Calculated). |
| 蛋白号: | Q9UBE0 |
| RRID: | AB_2833440 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0266, RRID:AB_2833440.
展开/折叠
Activator of SUMO1; AOS1; HSPC140; Sae1; SAE1_HUMAN; Sentrin/SUMO activating protein AOS1; SUA1; SUMO 1 activating enzyme E1 N subunit; SUMO 1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; Ubiquitin like protein SUMO1 activating enzyme; Ubiquitin-like 1-activating enzyme E1A; UBL E1A; UBLE1A;
抗原和靶标
Expression level increases during S phase and drops in G2 phase (at protein level).
- Q9UBE0 SAE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UBE0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| V2 | Acetylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Sumoylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K198 | Acetylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K228 | Acetylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot |
研究背景
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Nucleus.
Expression level increases during S phase and drops in G2 phase (at protein level).
Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.
Belongs to the ubiquitin-activating E1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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