Blooms Syndrome Protein Blm Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	Blooms Syndrome Protein Blm 抗体
货号:	AF6949
描述:	Rabbit polyclonal antibody to Blooms Syndrome Protein Blm
应用:	ELISA(peptide)
反应:	Human, Mouse, Rat
分子量:	159kD(Calculated).
蛋白号:	P54132
RRID:	AB_2847739

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

Blooms Syndrome Protein Blm Antibody detects endogenous levels of total Blooms Syndrome Protein Blm.

RRID:

AB_2847739
引用格式: Affinity Biosciences Cat# AF6949, RRID:AB_2847739.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Blm; BLM_HUMAN; Bloom syndrome; Bloom syndrome protein; Bloom syndrome RecQ helicase like; BS; DNA helicase; DNA helicase RecQ like type 2; MGC126616; MGC131618; MGC131620; RECQ 2; RECQ like; RecQ like type 2; RecQ protein like 3; RecQ protein-like 3; RecQ-like type 2; RecQ2; RECQL 2; RECQL 3; RECQL2; RECQL3; type 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human Blooms Syndrome Protein Blm.

Uniprot:

P54132(BLM_HUMAN) >>Visit HPA database.

基因/基因ID:

BLM(641)

序列:

P54132 BLM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

翻译修饰 - P54132 作为底物

P54132

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
S17	Phosphorylation		Uniprot
T20	Phosphorylation		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
S26	Phosphorylation		Uniprot
S28	Phosphorylation		Uniprot
K31	Sumoylation		Uniprot
S33	Phosphorylation		Uniprot
T36	Phosphorylation		Uniprot
K38	Methylation		Uniprot
K39	Methylation		Uniprot
K40	Sumoylation		Uniprot
S43	Phosphorylation		Uniprot
S48	Phosphorylation		Uniprot
T50	Phosphorylation		Uniprot
S53	Phosphorylation		Uniprot
K56	Sumoylation		Uniprot
T57	Phosphorylation		Uniprot
T68	Phosphorylation		Uniprot
S72	Phosphorylation		Uniprot
S74	Phosphorylation		Uniprot
K91	Sumoylation		Uniprot
K91	Ubiquitination		Uniprot
T99	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
K105	Sumoylation		Uniprot
K105	Ubiquitination		Uniprot
T114	Phosphorylation		Uniprot
T122	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
T125	Phosphorylation		Uniprot
T127	Phosphorylation		Uniprot
K129	Sumoylation		Uniprot
K129	Ubiquitination		Uniprot
S131	Phosphorylation		Uniprot
S142	Phosphorylation		Uniprot
S143	Phosphorylation		Uniprot
S144	Phosphorylation	P33981 (TTK)	Uniprot
K167	Ubiquitination		Uniprot
S168	Phosphorylation		Uniprot
T171	Phosphorylation	P49841 (GSK3B)	Uniprot
S175	Phosphorylation	P24941 (CDK2)	Uniprot
T182	Phosphorylation	O96017 (CHEK2) , O14757 (CHEK1)	Uniprot
K195	Methylation		Uniprot
K195	Sumoylation		Uniprot
Y199	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
K225	Ubiquitination		Uniprot
K259	Ubiquitination		Uniprot
S269	Phosphorylation		Uniprot
S282	Phosphorylation		Uniprot
Y296	Phosphorylation		Uniprot
S304	Phosphorylation		Uniprot
K317	Sumoylation		Uniprot
K323	Ubiquitination		Uniprot
T327	Phosphorylation		Uniprot
S328	Phosphorylation		Uniprot
K331	Sumoylation		Uniprot
K331	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot
S338	Phosphorylation	Q8NG66 (NEK11)	Uniprot
K339	Ubiquitination		Uniprot
S343	Phosphorylation		Uniprot
K344	Sumoylation		Uniprot
K344	Ubiquitination		Uniprot
K347	Sumoylation		Uniprot
S349	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
T359	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
K390	Ubiquitination		Uniprot
K392	Sumoylation		Uniprot
K409	Sumoylation		Uniprot
K409	Ubiquitination		Uniprot
T412	Phosphorylation		Uniprot
K418	Sumoylation		Uniprot
K418	Ubiquitination		Uniprot
S419	Phosphorylation		Uniprot
S422	Phosphorylation		Uniprot
S426	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
S443	Phosphorylation		Uniprot
S449	Phosphorylation		Uniprot
S456	Phosphorylation		Uniprot
S462	Phosphorylation		Uniprot
S464	Phosphorylation		Uniprot
T473	Phosphorylation		Uniprot
K476	Ubiquitination		Uniprot
S480	Phosphorylation		Uniprot
K484	Sumoylation		Uniprot
K484	Ubiquitination		Uniprot
K498	Sumoylation		Uniprot
S499	Phosphorylation		Uniprot
S502	Phosphorylation		Uniprot
T508	Phosphorylation		Uniprot
K514	Sumoylation		Uniprot
K514	Ubiquitination		Uniprot
K531	Ubiquitination		Uniprot
K535	Ubiquitination		Uniprot
T537	Phosphorylation		Uniprot
S539	Phosphorylation	O14757 (CHEK1)	Uniprot
S550	Phosphorylation		Uniprot
Y551	Phosphorylation		Uniprot
K578	Ubiquitination		Uniprot
S579	Phosphorylation		Uniprot
K588	Sumoylation		Uniprot
K588	Ubiquitination		Uniprot
K594	Sumoylation		Uniprot
K594	Ubiquitination		Uniprot
S601	Phosphorylation		Uniprot
K604	Ubiquitination		Uniprot
K629	Ubiquitination		Uniprot
S646	Phosphorylation	O14757 (CHEK1) , PR:O14757 (hCHEK1)	Uniprot
K653	Ubiquitination		Uniprot
K657	Acetylation		Uniprot
K661	Acetylation		Uniprot
K662	Acetylation		Uniprot
K662	Sumoylation		Uniprot
K662	Ubiquitination		Uniprot
Y699	Phosphorylation		Uniprot
S714	Phosphorylation	P06493 (CDK1)	Uniprot
Y736	Phosphorylation		Uniprot
K741	Ubiquitination		Uniprot
K755	Ubiquitination		Uniprot
T766	Phosphorylation	P06493 (CDK1)	Uniprot
K769	Ubiquitination		Uniprot
S773	Phosphorylation		Uniprot
K820	Sumoylation		Uniprot
K820	Ubiquitination		Uniprot
T830	Phosphorylation		Uniprot
T832	Phosphorylation		Uniprot
K839	Sumoylation		Uniprot
K839	Ubiquitination		Uniprot
K863	Acetylation		Uniprot
K863	Ubiquitination		Uniprot
K873	Sumoylation		Uniprot
K873	Ubiquitination		Uniprot
T903	Phosphorylation		Uniprot
T907	Phosphorylation		Uniprot
K1057	Ubiquitination		Uniprot
K1082	Sumoylation		Uniprot
K1082	Ubiquitination		Uniprot
S1083	Phosphorylation		Uniprot
K1125	Sumoylation		Uniprot
K1125	Ubiquitination		Uniprot
K1133	Ubiquitination		Uniprot
S1197	Phosphorylation		Uniprot
K1199	Sumoylation		Uniprot
K1199	Ubiquitination		Uniprot
K1207	Ubiquitination		Uniprot
K1217	Ubiquitination		Uniprot
K1273	Sumoylation		Uniprot
K1273	Ubiquitination		Uniprot
S1280	Phosphorylation		Uniprot
S1290	Phosphorylation	P33981 (TTK)	Uniprot
S1295	Phosphorylation		Uniprot
S1296	Phosphorylation	P33981 (TTK)	Uniprot
S1303	Phosphorylation		Uniprot
S1304	Phosphorylation		Uniprot
S1310	Phosphorylation		Uniprot
K1329	Ubiquitination		Uniprot
K1372	Sumoylation		Uniprot
K1372	Ubiquitination		Uniprot
S1375	Phosphorylation		Uniprot
S1383	Phosphorylation		Uniprot
K1411	Acetylation		Uniprot
S1417	Phosphorylation		Uniprot

研究背景

P54132_BLM_HUMAN

功能:

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction.

翻译修饰:

Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.

细胞定位:

Nucleus.
Note: Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner.

亚基结构:

Monomer. Homodimer (via N-terminus). Homotetramer (via N-terminus); dimer of dimers. Homohexamer (via N-terminus). Self-association negatively regulates DNA unwinding amplitude and rate. Oligomeric complexes dissociate into monomer in presence of ATP. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2. Interacts with RMI complex. Interacts directly with RMI1 (via N-terminal region) component of RMI complex. Interacts with SUPV3L1. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with TOP3A (via N-terminal region). Interacts with SPIDR (via C-terminal region); the interaction is direct and required to target BLM to sites of DNA damage.

蛋白家族:

The N-terminal region mediates dimerization and homooligomerization (PubMed:28228481). Both the helicase ATP-binding domain and the helicase C-terminal domain form intramolecular interactions with the HRDC domain in a ATP-dependent manner (PubMed:25901030). The HRDC domain is required for single-stranded DNA (ssDNA) and DNA Holliday junction binding (PubMed:20639533).

Belongs to the helicase family. RecQ subfamily.

研究领域

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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Blooms Syndrome Protein Blm Antibody - #AF6949

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