VAV1 Antibody - #AF6182

Figure 1 Increased Vav1 expression in the ischemic penumbra of MCAO/R rats.(A) Vav1 mRNA expression in MCAO/R rats according to the GEO database GSE163614. (B) Vav1 mRNA expression (normalized to the sham group) in the ischemic penumbra of MCAO/R rats. (C) Normalized Vav1 protein expression in the ischemic penumbra of MCAO/R rats. (D) Cerebral infarction area expressed as a percentage of total brain area, as determined by TTC staining. Data are expressed as the mean ± SD (n = 6), and were analyzed by unpaired t-test. The experiments were repeated three times. GEO: Gene expression omnibus; MCAO/R: middle cerebral artery occlusion/reperfusion; TTC staining: triphenyltetrazolium chloride staining; Vav1: Vav guanine nucleotide exchange factor 1. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023.

Figure 4 Vav1 knockdown represses the activation of microglia and NLRP3 inflammasome in the ischemic penumbra of MCAO/R rats.(A) Double immunofluorescence staining for Vav1 (FITC, green) and Iba-1 (Cy3, red) in the ischemic penumbra area in MCAO/R rats. MCAO/R rats showed enhanced expression of Vav1 and Iba-1 compared with the sham group, but inhibition of Vav1 expression decreased the levels of both proteins. (B) Quantification of Vav1/Iba-1 double positive cells. (C) Double immunofluorescence staining for NLRP3 (FITC, green) and Iba-1 (Cy3, red) in the ischemic penumbra area in MCAO/R rats. MCAO/R rats showed enhanced expression of Iba-1 and NLRP3 compared with the sham group, but inhibition of Vav1 expression decreased the levels of both proteins. Nuclei were stained with DAPI (blue). Scale bars: 50 μm. (D) Quantification of NLRP3 and Iba-1 double positive cells. Data are expressed as the mean ± SD (n = 6), and were analyzed by one-way analysis of variance followed by Tukey’s multiple comparisons test. Cy3: Cyanine 3; DAPI: 4′,6-diamidino-2-phenylindole; FITC: fluorescein isothiocyanate; MCAO/R: middle cerebral artery occlusion/reperfusion; NLRP3: NOD-like receptor pyrin 3; Vav1: Vav guanine nucleotide exchange factor 1. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023.