Rictor Antibody - #AF2697

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (2)  
产品: Rictor 抗体
货号: AF2697
描述: Rabbit polyclonal antibody to Rictor
应用: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 200kDa; 192kD(Calculated).
蛋白号: Q6R327
RRID: AB_2847770

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
克隆:
Polyclonal
特异性:
Rictor Antibody detects endogenous levels of total Rictor.
RRID:
AB_2847770
引用格式: Affinity Biosciences Cat# AF2697, RRID:AB_2847770.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin(Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae); mAVO3; MGC39830; PIA; Pianissimo; Rapamycin insensitive companion of mTOR; Rapamycin-insensitive companion of mTOR; Rictor; RICTR; RICTR_HUMAN; RPTOR independent companion of MTOR complex 2; TORC2 specific protein AVO3;

抗原和靶标

免疫原:
Uniprot:

Q6R327(RICTR_HUMAN) >>Visit HPA database.

基因/基因ID:
RICTOR(253260)
序列:
MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAES

翻译修饰 - Q6R327 作为底物

Site PTM Type Enzyme
R6 Methylation
S21 Phosphorylation
S35 Phosphorylation
K56 Ubiquitination
K65 Ubiquitination
K97 Ubiquitination
Y109 Phosphorylation
K213 Acetylation
Y243 Phosphorylation
S265 Phosphorylation
K274 Ubiquitination
S284 Phosphorylation
K374 Ubiquitination
S383 Phosphorylation
T424 Phosphorylation
K477 Ubiquitination
K507 Ubiquitination
K516 Ubiquitination
K522 Ubiquitination
K566 Ubiquitination
K582 Acetylation
K719 Ubiquitination
T733 Phosphorylation
K764 Ubiquitination
K781 Ubiquitination
K791 Ubiquitination
K813 Ubiquitination
Y823 Phosphorylation
K826 Ubiquitination
K830 Ubiquitination
Y835 Phosphorylation
S837 Phosphorylation
K856 Ubiquitination
Y863 Phosphorylation
K916 Ubiquitination
K921 Ubiquitination
K979 Ubiquitination
K986 Methylation
K986 Ubiquitination
S1026 Phosphorylation
S1028 Phosphorylation
S1030 Phosphorylation
S1031 Phosphorylation
S1035 Phosphorylation
S1037 Phosphorylation
Y1073 Phosphorylation
K1082 Ubiquitination
S1084 Phosphorylation
S1090 Phosphorylation
K1092 Ubiquitination
S1101 Phosphorylation
T1103 Phosphorylation
K1107 Ubiquitination
K1116 Acetylation
K1119 Acetylation
K1125 Acetylation
K1125 Ubiquitination
T1133 Phosphorylation
T1135 Phosphorylation Q9UBS0 (RPS6KB2) , P31749 (AKT1) , O00141 (SGK1) , P23443 (RPS6KB1)
S1138 Phosphorylation
S1144 Phosphorylation
T1148 Phosphorylation
T1161 Phosphorylation
S1162 Phosphorylation
K1167 Ubiquitination
T1172 Phosphorylation
S1174 Phosphorylation
T1175 Phosphorylation
S1177 Phosphorylation
K1184 Ubiquitination
K1187 Ubiquitination
S1211 Phosphorylation
S1217 Phosphorylation
S1219 Phosphorylation
T1222 Phosphorylation
T1224 Phosphorylation
T1225 Phosphorylation
T1226 Phosphorylation
S1227 Phosphorylation
S1230 Phosphorylation
S1231 Phosphorylation
S1234 Phosphorylation
S1235 Phosphorylation P49841 (GSK3B)
K1265 Ubiquitination
S1267 Phosphorylation
Y1269 Phosphorylation
T1271 Phosphorylation
S1274 Phosphorylation
S1278 Phosphorylation
K1281 Ubiquitination
S1282 Phosphorylation
S1284 Phosphorylation
S1286 Phosphorylation
S1292 Phosphorylation
S1293 Phosphorylation
T1295 Phosphorylation
S1302 Phosphorylation
K1304 Methylation
K1304 Ubiquitination
S1307 Phosphorylation
T1310 Phosphorylation
K1312 Acetylation
K1312 Methylation
K1312 Ubiquitination
S1313 Phosphorylation
S1320 Phosphorylation
Y1321 Phosphorylation
Y1330 Phosphorylation
T1332 Phosphorylation
K1334 Methylation
K1334 Ubiquitination
S1344 Phosphorylation
S1346 Phosphorylation
S1348 Phosphorylation
S1353 Phosphorylation
T1361 Phosphorylation
K1367 Methylation
S1370 Phosphorylation
S1373 Phosphorylation
T1376 Phosphorylation
K1382 Ubiquitination
S1385 Phosphorylation
Y1386 Phosphorylation
S1388 Phosphorylation
K1391 Ubiquitination
S1396 Phosphorylation
T1402 Phosphorylation
S1406 Phosphorylation
S1407 Phosphorylation
S1411 Phosphorylation
S1414 Phosphorylation
S1415 Phosphorylation
T1417 Phosphorylation
Y1418 Phosphorylation
S1421 Phosphorylation
Y1444 Phosphorylation
T1447 Phosphorylation
K1448 Ubiquitination
S1470 Phosphorylation
T1472 Phosphorylation
K1477 Ubiquitination
S1479 Phosphorylation
S1571 Phosphorylation
S1574 Phosphorylation
S1577 Phosphorylation
S1581 Phosphorylation
S1585 Phosphorylation
K1590 Ubiquitination
S1591 Phosphorylation
T1592 Phosphorylation
K1599 Ubiquitination
T1605 Phosphorylation
K1640 Ubiquitination
T1695 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)

研究背景

功能:

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

翻译修饰:

Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.

亚基结构:

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex. Interacts with CCDC28B. Interacts with NBN. Interacts with PRR5L. Interacts with SIK3.

(Microbial infection) Interacts with vaccinia virus protein F17; this interaction dysregulates mTOR.

蛋白家族:

Belongs to the RICTOR family.

研究领域

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

文献引用

1). Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget, 2017 (PubMed: 29179463)

Application: WB    Species: mouse    Sample: Sertoli cells

Figure 4:| OLA attenuates mTORC2 complex activity. Sertoli cells cultured on dishes were treated on day 3 with 100-, 200-, 400- or 800 μg/ml OLA for 24 h. Cells treated with vehicle (0.2% DMSO) were used as negative control. Thereafter, cells were washed twice with PBS to remove residual OLA and terminated for immunoblot (IB). Immunoblot analysis to assess the effects of OLA on the expression of mTORC2 complex: Ritor and p-mTOR, downstream proteins: PKC, Akt, p-PKC and p-AktSer473. GAPDH served as protein loading control. Semiquantitative analysis of protein expression in following histogram (mean ± S.E.M., three independent replicates per groups). * p < 0.05; ** p < 0.01.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.