产品: MAP4 抗体
货号: AF0079
描述: Rabbit polyclonal antibody to MAP4
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 121kDa,200kDa; 121kD(Calculated).
蛋白号: P27816
RRID: AB_2847896

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
MAP4 Antibody detects endogenous levels of total MAP4.
RRID:
AB_2847896
引用格式: Affinity Biosciences Cat# AF0079, RRID:AB_2847896.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730;

抗原和靶标

免疫原:

A synthesized peptide derived from human MAP4.

Uniprot:
序列:
MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI

翻译修饰 - P27816 作为底物

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
T11 Phosphorylation
S14 Phosphorylation
T28 Phosphorylation
T41 Phosphorylation
T45 Phosphorylation
Y47 Phosphorylation
K56 Ubiquitination
T57 Phosphorylation
S60 Phosphorylation
S68 Phosphorylation
S71 Phosphorylation
T76 Phosphorylation
S94 Phosphorylation
T96 Phosphorylation
T97 Phosphorylation
S99 Phosphorylation
T101 Phosphorylation
Y110 Phosphorylation
Y113 Phosphorylation
S116 Phosphorylation
T183 Phosphorylation
S197 Phosphorylation
S200 Phosphorylation
S202 Phosphorylation
S230 Phosphorylation
K254 Ubiquitination
T256 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K269 Acetylation
K269 Sumoylation
K269 Ubiquitination
K276 Acetylation
S280 Phosphorylation
T282 Phosphorylation
T287 Phosphorylation
S295 Phosphorylation
K312 Ubiquitination
K318 Ubiquitination
T324 Phosphorylation
T326 Phosphorylation
S329 Phosphorylation
S330 Phosphorylation
K332 Ubiquitination
T340 Phosphorylation
K346 Acetylation
K346 Ubiquitination
K352 Ubiquitination
T354 Phosphorylation
S358 Phosphorylation
K361 Ubiquitination
S367 Phosphorylation
K368 Acetylation
K368 Ubiquitination
T380 Phosphorylation
S384 Phosphorylation
K387 Ubiquitination
S393 Phosphorylation
K394 Acetylation
K394 Ubiquitination
K400 Ubiquitination
S439 Phosphorylation
S440 Phosphorylation
T442 Phosphorylation
T451 Phosphorylation
K464 Ubiquitination
K476 Acetylation
K476 Ubiquitination
T484 Phosphorylation
K490 Ubiquitination
S495 Phosphorylation
T496 Phosphorylation
K498 Ubiquitination
S507 Phosphorylation
S510 Phosphorylation
T512 Phosphorylation
T521 Phosphorylation
T526 Phosphorylation
K560 Ubiquitination
T565 Phosphorylation
T571 Phosphorylation
K574 Ubiquitination
S580 Phosphorylation
T582 Phosphorylation
T585 Phosphorylation
S602 Phosphorylation
S605 Phosphorylation
S609 Phosphorylation
T620 Phosphorylation
S624 Phosphorylation
T627 Phosphorylation
K633 Acetylation
K634 Ubiquitination
C635 S-Nitrosylation
S636 Phosphorylation
S643 Phosphorylation
K647 Ubiquitination
T686 Phosphorylation
T687 Phosphorylation
S696 Phosphorylation P06493 (CDK1)
K702 Acetylation
K702 Ubiquitination
T706 Phosphorylation
T712 Phosphorylation
S713 Phosphorylation
K718 Acetylation
T719 Phosphorylation
T722 Phosphorylation
S723 Phosphorylation
K726 Ubiquitination
T731 Phosphorylation
S742 Phosphorylation
S745 Phosphorylation
S760 Phosphorylation
S764 Phosphorylation
S768 Phosphorylation
K784 Acetylation
S787 Phosphorylation P06493 (CDK1)
S789 Phosphorylation
S793 Phosphorylation
S797 O-Glycosylation
S797 Phosphorylation
S803 Phosphorylation
T804 Phosphorylation
T806 Phosphorylation
S818 Phosphorylation
T819 Phosphorylation
S822 Phosphorylation
S823 Phosphorylation
S825 Phosphorylation
S827 Phosphorylation
T828 Phosphorylation
K838 Acetylation
K838 Sumoylation
K847 Acetylation
K852 Acetylation
S853 Phosphorylation
S859 Phosphorylation
S863 Phosphorylation
S865 Phosphorylation
S868 Phosphorylation
K871 Acetylation
T872 Phosphorylation
T874 Phosphorylation
S876 Phosphorylation
T878 Phosphorylation
T892 Phosphorylation P06493 (CDK1)
S896 Phosphorylation
S899 Phosphorylation
T900 Phosphorylation
T901 Phosphorylation P06493 (CDK1)
T909 Phosphorylation
K912 Acetylation
S914 Phosphorylation
S915 Phosphorylation
T917 Phosphorylation P06493 (CDK1)
T925 Phosphorylation O14757 (CHEK1)
T927 Phosphorylation
S928 Phosphorylation
K933 Acetylation
K933 Ubiquitination
S937 Phosphorylation
K938 Acetylation
S941 Phosphorylation Q9P0L2 (MARK1)
T942 Phosphorylation
K946 Ubiquitination
K955 Methylation
T960 Phosphorylation
T965 Phosphorylation
T977 Phosphorylation
S983 Phosphorylation
K986 Ubiquitination
K991 Acetylation
K991 Ubiquitination
S996 Phosphorylation
K997 Acetylation
K998 Acetylation
K998 Ubiquitination
S1000 Phosphorylation
Y1001 Phosphorylation
S1002 Phosphorylation
S1006 Phosphorylation
K1007 Ubiquitination
S1010 Phosphorylation
K1011 Methylation
K1015 Ubiquitination
K1028 Acetylation
K1028 Ubiquitination
K1034 Acetylation
S1036 Phosphorylation
S1037 Phosphorylation
K1038 Acetylation
S1041 Phosphorylation
K1042 Acetylation
S1058 Phosphorylation
K1060 Acetylation
K1060 Ubiquitination
K1064 Methylation
K1070 Acetylation
K1070 Ubiquitination
S1073 Phosphorylation Q9P0L2 (MARK1)
T1142 Phosphorylation
S1145 Phosphorylation
T1150 Phosphorylation
S1151 Phosphorylation

研究背景

功能:

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

翻译修饰:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with SEPTIN2; this interaction impedes tubulin-binding. Interacts with TRAF3IP1 (By similarity).

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.