| 产品: | MAP4 抗体 |
| 货号: | AF0079 |
| 描述: | Rabbit polyclonal antibody to MAP4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 121kDa,200kDa; 121kD(Calculated). |
| 蛋白号: | P27816 |
| RRID: | AB_2847896 |
产品描述
来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
MAP4 Antibody detects endogenous levels of total MAP4.
RRID:
AB_2847896
引用格式: Affinity Biosciences Cat# AF0079, RRID:AB_2847896.
引用格式: Affinity Biosciences Cat# AF0079, RRID:AB_2847896.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730;
抗原和靶标
免疫原:
A synthesized peptide derived from human MAP4.
Uniprot:
序列:
- P27816 MAP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI
翻译修饰 - P27816 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K269 | Acetylation | Uniprot | |
| K269 | Sumoylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K276 | Acetylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| T287 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K368 | Acetylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| K476 | Acetylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| T484 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| T496 | Phosphorylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| T512 | Phosphorylation | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| T571 | Phosphorylation | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| T582 | Phosphorylation | Uniprot | |
| T585 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| T620 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T627 | Phosphorylation | Uniprot | |
| K633 | Acetylation | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| C635 | S-Nitrosylation | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| T686 | Phosphorylation | Uniprot | |
| T687 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K702 | Acetylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| T706 | Phosphorylation | Uniprot | |
| T712 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K718 | Acetylation | Uniprot | |
| T719 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| T731 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S760 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| K784 | Acetylation | Uniprot | |
| S787 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S789 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| S797 | O-Glycosylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| T804 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| T819 | Phosphorylation | Uniprot | |
| S822 | Phosphorylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S827 | Phosphorylation | Uniprot | |
| T828 | Phosphorylation | Uniprot | |
| K838 | Acetylation | Uniprot | |
| K838 | Sumoylation | Uniprot | |
| K847 | Acetylation | Uniprot | |
| K852 | Acetylation | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| S859 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| S868 | Phosphorylation | Uniprot | |
| K871 | Acetylation | Uniprot | |
| T872 | Phosphorylation | Uniprot | |
| T874 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T878 | Phosphorylation | Uniprot | |
| T892 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S896 | Phosphorylation | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| T900 | Phosphorylation | Uniprot | |
| T901 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T909 | Phosphorylation | Uniprot | |
| K912 | Acetylation | Uniprot | |
| S914 | Phosphorylation | Uniprot | |
| S915 | Phosphorylation | Uniprot | |
| T917 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T925 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T927 | Phosphorylation | Uniprot | |
| S928 | Phosphorylation | Uniprot | |
| K933 | Acetylation | Uniprot | |
| K933 | Ubiquitination | Uniprot | |
| S937 | Phosphorylation | Uniprot | |
| K938 | Acetylation | Uniprot | |
| S941 | Phosphorylation | Q9P0L2 (MARK1) | Uniprot |
| T942 | Phosphorylation | Uniprot | |
| K946 | Ubiquitination | Uniprot | |
| K955 | Methylation | Uniprot | |
| T960 | Phosphorylation | Uniprot | |
| T965 | Phosphorylation | Uniprot | |
| T977 | Phosphorylation | Uniprot | |
| S983 | Phosphorylation | Uniprot | |
| K986 | Ubiquitination | Uniprot | |
| K991 | Acetylation | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| S996 | Phosphorylation | Uniprot | |
| K997 | Acetylation | Uniprot | |
| K998 | Acetylation | Uniprot | |
| K998 | Ubiquitination | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| Y1001 | Phosphorylation | Uniprot | |
| S1002 | Phosphorylation | Uniprot | |
| S1006 | Phosphorylation | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| S1010 | Phosphorylation | Uniprot | |
| K1011 | Methylation | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| K1028 | Acetylation | Uniprot | |
| K1028 | Ubiquitination | Uniprot | |
| K1034 | Acetylation | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1037 | Phosphorylation | Uniprot | |
| K1038 | Acetylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot | |
| K1042 | Acetylation | Uniprot | |
| S1058 | Phosphorylation | Uniprot | |
| K1060 | Acetylation | Uniprot | |
| K1060 | Ubiquitination | Uniprot | |
| K1064 | Methylation | Uniprot | |
| K1070 | Acetylation | Uniprot | |
| K1070 | Ubiquitination | Uniprot | |
| S1073 | Phosphorylation | Q9P0L2 (MARK1) | Uniprot |
| T1142 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| T1150 | Phosphorylation | Uniprot | |
| S1151 | Phosphorylation | Uniprot |
研究背景
功能:
Non-neuronal microtubule-associated protein. Promotes microtubule assembly.
翻译修饰:
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
细胞定位:
Cytoplasm>Cytoskeleton.
亚基结构:
Interacts with SEPTIN2; this interaction impedes tubulin-binding. Interacts with TRAF3IP1 (By similarity).
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