(4)
(2)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
MYCD; MYCD_HUMAN; Myocardin; Myocd;
抗原和靶标
A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.
Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
- Q8IZQ8 MYCD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW
翻译修饰 - Q8IZQ8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S74 | Phosphorylation | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| K264 | Methylation | Uniprot | |
| S451 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S455 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S459 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S463 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| K571 | Sumoylation | Uniprot | |
| K588 | Acetylation | Uniprot | |
| S626 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S630 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S634 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S638 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S704 | Phosphorylation | Uniprot | |
| T732 | Phosphorylation | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S862 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S869 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T896 | Phosphorylation | P28482 (MAPK1) | Uniprot |
研究背景
Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).
Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.
Nucleus.
Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).
The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).
文献引用
Application: WB Species: mice Sample: cardiomyocytes
Application: WB Species: mouse Sample: primary cardiomyocytes
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