ONECUT2 Antibody - #DF13779

Figure 1. ONECUT2 is an oncogene in gastric cancer. (a) LIMMA R package was used for analyzing differential gene expression of STAD samples and adjacent normal tissues in TCGA Database. ONECUT2 and DEGs whose absLogFC ranked top 10 were exhibited in a histogram. P value < 0.001. (b) qPCR analysis of hsa-miR-15a-5p expression between STAD samples and adjacent normal tissues. (c, d) Hsa-miR-15a-5p expression in 30 pairs of STAD samples and their corresponding non-tumorous tissues. (e, f) Immunohistochemistry detected the expression and distribution of ONECUT2 in the STAD samples and adjacent normal tissues and photos were taken. (g, h) GEPIA website analyzed the correlation between ONECUT2 expression and the survival of STAD patients. (A color version of this figure is available in the online journal.)

Figure 4. Hsa-miR-15a-5p regulates the transcription and translation of ONECUT2 in GCSCs. (a) qPCR analysis of hsa-miR-15a-5p and ONECUT2 in human gastric adenocarcinoma cell lines HGC-27, AGS, SGC-7901, MKN45 and normal human gastric mucosal epithelial cell line GES-1. *P<0.05, ***P<0.001, ##P<0.01, ###P<0.001, ∼ versus GES-1. (b) qPCR analysis of hsa-miR-15a-5p and ONECUT2 in parental, CD133+/CD44+ and CD133-/CD44- MKN45. **P<0.01, ###P<0.001, ∼ versus parental MKN45. (c) Western blot detected ONECUT2 expression in parental, CD133+/CD44+ and CD133-/CD44- MKN45. (d) Western blot detected ONECUT2 expression in CD133+/CD44+ MKN45 transfected with hsa-miR-15a-5p mimics, and in CD133-/CD44- MKN45 transfected with hsa-miR-15a-5p inhibitors. (e) The sequence of hsa-miR-15a-5p and its target ONECUT2. (f) We constructed pGL3-ONECUT2-WT and pGL3-ONECUT2-MUT. Then, CD133+/CD44+ MKN45 were transfected with hsa-miR-15a-5p mimics. (g, h) qPCR and Western blot verified the efficiency of ONECUT2 overexpression (OE) vector. (A color version of this figure is available in the online journal.)