产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6205, RRID:AB_2835086.
展开/折叠
PTP1B; Non receptor tyrosine phosphatase 1; Protein phosphotyrosylphosphatase 1B; Protein tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; Protein-tyrosine phosphatase 1B; PTN1_HUMAN; PTP 1B; PTP-1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1; Tyrosine-protein phosphatase non-receptor type 1;
抗原和靶标
- P18031 PTN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P18031 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| C32 | S-Nitrosylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P49759 (CLK1) , P31749 (AKT1) , P49760 (CLK2) | Uniprot |
| S55 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | P00533 (EGFR) , P06213 (INSR) | Uniprot |
| S70 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| C92 | S-Nitrosylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | P06213 (INSR) | Uniprot |
| Y153 | Phosphorylation | P06213 (INSR) | Uniprot |
| S201 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| C215 | S-Nitrosylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | P49759 (CLK1) , P49760 (CLK2) | Uniprot |
| S243 | Phosphorylation | P49759 (CLK1) , P49760 (CLK2) | Uniprot |
| K247 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S295 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K335 | Sumoylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| K347 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| Y359 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | P68400 (CSNK2A1) , P17252 (PRKCA) , P19784 (CSNK2A2) | Uniprot |
| R380 | Methylation | Uniprot | |
| S386 | Phosphorylation | P06493 (CDK1) , P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S393 | Phosphorylation | P53350 (PLK1) | Uniprot |
研究背景
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Interacts with EPHA3 at the cell membrane.
Expressed in keratinocytes (at protein level).
Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
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