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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6243, RRID:AB_2835107.
展开/折叠
CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; Dual specificity phosphatase 4; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; DUS4_HUMAN; DUSP 4; Dusp4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; Mitogen-activated protein kinase phosphatase 2; MKP 2; MKP-2; MKP2; Serine/threonine specific protein phosphatase; TYP; Typ1; VH 2; VH1 homologous phosphatase 2; VH2;
抗原和靶标
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
- P28562 DUS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC
- Q13115 DUS4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P28562/Q13115 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K57 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K57 | Ubiquitination | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S323 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S334 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S359 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S364 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| V2 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| Y154 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot |
研究背景
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
Nucleus.
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.
Nucleus.
Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
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