| 产品: | GGA1 抗体 |
| 货号: | DF14339 |
| 描述: | Rabbit polyclonal antibody to GGA1 |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse |
| 分子量: | 70kD(Calculated). |
| 蛋白号: | Q9UJY5 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
4930406E12Rik; ADP ribosylation factor binding protein 1; ADP ribosylation factor binding protein GGA1; ADP-ribosylation factor-binding protein GGA1; ARF binding protein 1; ARF-binding protein 1; Gamma adaptin related protein 1; gamma ear-containing; Gamma-adaptin-related protein 1; GGA 1; GGA1; GGA1 protein; GGA1_HUMAN; Golgi associated gamma adaptin ear containing ARF; Golgi associated gamma adaptin ear containing ARF binding protein 1; Golgi localized gamma ear containing ARF binding protein 1; Golgi-localized; OTTHUMP00000028975; OTTHUMP00000042200;
抗原和靶标
A synthesized peptide derived from Human GGA1.
- Q9UJY5 GGA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQANDNLTQVINLYKQLVRGEEVNGDATAGSIPGSTSALLDLSGLDLPPAGTTYPAMPTRPGEQASPEQPSASVSLLDDELMSLGLSDPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGLDDLDLLGKTLLQQSLPPESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQPVPTELSLASITVPLESIKPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFPPPETWGSL
研究背景
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein.
Ubiquitously expressed.
Monomer (Probable). Interacts with GGA2 and GGA3. Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6. Interacts with RABEP1 and RABGEF1. Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain). Interacts with EPN4. Binds CCDC91, P200 and SYNRG. Interacts with NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Interacts with RNF11. Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498'. Interacts with CNST (By similarity). Interacts with ADRA2B. Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (By similarity).
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Belongs to the GGA protein family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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