(2)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6291, RRID:AB_2835141.
展开/折叠
CD130; CD130 antigen; CDw130; gp130; GP130 RAPS; IL 6R beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6 ST; IL6RB_HUMAN; IL6ST; Interleukin 6 receptor subunit beta; Interleukin receptor beta chain; Interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; Membrane glycoprotein gp130; Oncostatin M receptor; Oncostatin M receptor alpha subunit; Oncostatin-M receptor subunit alpha;
抗原和靶标
Found in all the tissues and cell lines examined (PubMed:2261637). Expression not restricted to IL6 responsive cells (PubMed:2261637). Expressed in blood serum (at protein level) (PubMed:24629561).
- P40189 IL6RB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLTLQTWLVQALFIFLTTESTGELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEAIVVPVCLAFLLTTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDGNFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSDENESSQNTSSTVQYSTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMPQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P40189 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N43 | N-Glycosylation | Uniprot | |
| N83 | N-Glycosylation | Uniprot | |
| N131 | N-Glycosylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| N157 | N-Glycosylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| N227 | N-Glycosylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| N379 | N-Glycosylation | Uniprot | |
| N383 | N-Glycosylation | Uniprot | |
| N390 | N-Glycosylation | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| N553 | N-Glycosylation | Uniprot | |
| N564 | N-Glycosylation | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| T670 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| Y683 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| Y759 | Phosphorylation | Uniprot | |
| T761 | Phosphorylation | Uniprot | |
| Y767 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| T783 | Phosphorylation | Uniprot | |
| Y814 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S824 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| K849 | Methylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| T890 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| T897 | Phosphorylation | Uniprot | |
| Y905 | Phosphorylation | Uniprot | |
| T909 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| Y915 | Phosphorylation | Uniprot |
研究背景
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.
Cell membrane>Single-pass type I membrane protein.
Secreted.
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Expressed in blood serum (at protein level).
Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Forms heterodimers composed of LIFR and IL6ST (type I OSM receptor) which are activated by LIF and OSM. Also forms heterodimers composed of OSMR and IL6ST (type II receptor) which are activated by OSM but not by LIF. Homodimer. Interacts with HCK. Interacts with INPP5D/SHIP1 (By similarity).
(Microbial infection) The homodimer binds two molecules of herpes virus 8/HHV-8 protein vIL-6.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Belongs to the type I cytokine receptor family. Type 2 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
文献引用
Application: WB Species: rat Sample: spinal cord
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