产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Alpha-tubulin 1; ALS22; B ALPHA 1; bA408E5.3; H2 ALPHA; Hum a tub1; Hum a tub2; LIS3; MGC171407; MGC55332; TBA4A_HUMAN; Testis-specific alpha-tubulin; TUBA1; TUBA1A; tuba1l; Tuba4a; Tubulin alpha 1 chain; Tubulin alpha; Tubulin alpha-1 chain; tubulin alpha-1B chain; Tubulin alpha-4A chain; Tubulin H2-alpha; Tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; Tubulin, alpha 4a; Tubulin, alpha, testis-specific; Tubulin, alpha-1;
抗原和靶标
A synthesized peptide derived from human TUBA4A .
- P68366 TBA4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
翻译修饰 - P68366 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| C54 | S-Nitrosylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Sumoylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K112 | Sumoylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K124 | Methylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K163 | Acetylation | Uniprot | |
| K163 | Methylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| C295 | S-Nitrosylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K311 | Acetylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K336 | Sumoylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| R339 | Methylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| C347 | S-Nitrosylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Sumoylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| K370 | Acetylation | Uniprot | |
| K370 | Sumoylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| C376 | S-Nitrosylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K394 | Sumoylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K401 | Sumoylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| Y408 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y432 | Phosphorylation | P43405 (SYK) | Uniprot |
| S439 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot |
研究背景
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.
Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Cytoplasm>Cytoskeleton.
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CFAP157 (By similarity).
Belongs to the tubulin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
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