AP2 alpha Antibody - #DF14475
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Activating enhancer binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP 2 transcription factor; AP 2alpha; AP-2; AP-2 transcription factor; AP2; AP2 Transcription Factor; AP2-alpha; AP2A_HUMAN; AP2TF; BOFS; FLJ51761; TFAP 2; TFAP 2A; TFAP2; TFAP2A; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha; Transcription factor AP2 alpha;
抗原和靶标
A synthesized peptide derived from human AP2 alpha.
- P05549 AP2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLWKLTDNIKYEDCEDRHDGTSNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHALSSGLGDLSIHSLPHAIEEVPHVEDPGINIPDQTVIKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLGNSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNNAKSSDKEEKHRK
翻译修饰 - P05549 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K10 | Sumoylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K177 | Sumoylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S258 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K268 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K335 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Sumoylated on Lys-10; which inhibits transcriptional activity.
Nucleus.
Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Interacts with KCTD15; this interaction inhibits TFAP2A transcriptional activation.
The PPxY motif mediates interaction with WWOX.
Belongs to the AP-2 family.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.