产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6301, RRID:AB_2835150.
展开/折叠
12S1644; D12S1644; IL 4 STAT; IL-4 Stat; IL4 STAT; Interleukin 4 Induced; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal transducer and activator of transcription 6; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1; Signal transducer and activator of transcription 6, interleukin 4 induced; STAT 6; STAT interleukin4 induced; STAT, interleukin4 induced; Stat6; STAT6_HUMAN; STAT6B; STAT6C; Transcription factor IL 4 STAT;
抗原和靶标
- P42226 STAT6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLWGLVSKMPPEKVQRLYVDFPQHLRHLLGDWLESQPWEFLVGSDAFCCNLASALLSDTVQHLQASVGEQGEGSTILQHISTLESIYQRDPLKLVATFRQILQGEKKAVMEQFRHLPMPFHWKQEELKFKTGLRRLQHRVGEIHLLREALQKGAEAGQVSLHSLIETPANGTGPSEALAMLLQETTGELEAAKALVLKRIQIWKRQQQLAGNGAPFEESLAPLQERCESLVDIYSQLQQEVGAAGGELEPKTRASLTGRLDEVLRTLVTSCFLVEKQPPQVLKTQTKFQAGVRFLLGLRFLGAPAKPPLVRADMVTEKQARELSVPQGPGAGAESTGEIINNTVPLENSIPGNCCSALFKNLLLKKIKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWDNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQIENIQPFSAKDLSIRSLGDRIRDLAQLKNLYPKKPKDEAFRSHYKPEQMGKDGRGYVPATIKMTVERDQPLPTPELQMPTMVPSYDLGMAPDSSMSMQLGPDMVPQVYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDLRANPSW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P42226 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| S407 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| K450 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| T525 | Phosphorylation | Uniprot | |
| K595 | Ubiquitination | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| Y641 | Phosphorylation | Q9UHD2 (TBK1) | Uniprot |
| T645 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| S707 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S756 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot |
研究背景
Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.
Mono-ADP-ribosylated by PARP14.
Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.
Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.
Belongs to the transcription factor STAT family.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
限制条款
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