产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6487, RRID:AB_2835167.
展开/折叠
DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B;
抗原和靶标
- Q9UD71 PPR1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREEDEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPPLDESERDGGSEDQVEDPALSEPGEEPQRPSPSEPGT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UD71 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S45 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S78 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot |
研究背景
Inhibitor of protein-phosphatase 1.
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
Cytoplasm.
Belongs to the protein phosphatase inhibitor 1 family.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Cocaine addiction.
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Nervous system > Dopaminergic synapse.
限制条款
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