SOX9 Antibody - #AF6330

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产品: SOX9 抗体
货号: AF6330
描述: Rabbit polyclonal antibody to SOX9
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat, Monkey
预测: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 56kDa; 56kD(Calculated).
蛋白号: P48436
RRID: AB_2835186

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ip 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
克隆:
Polyclonal
特异性:
SOX9 Antibody detects endogenous levels of total SOX9.
RRID:
AB_2835186
引用格式: Affinity Biosciences Cat# AF6330, RRID:AB_2835186.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

campomelic dysplasia autosomal sex reversal; CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; SRY related HMG box gene 9; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9;

抗原和靶标

免疫原:
Uniprot:

P48436(SOX9_HUMAN) >>Visit HPA database.

基因/基因ID:
SOX9(6662)
描述:
SOX9 Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Defects in SOX9 are the cause of campomelic dysplasia (CMD1).
序列:
MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48436 作为底物

Site PTM Type Enzyme
T11 Phosphorylation
S30 Phosphorylation
T43 Phosphorylation
T46 Phosphorylation
K61 Acetylation
S64 Phosphorylation P17612 (PRKACA)
K68 Ubiquitination
S78 Phosphorylation
K82 Ubiquitination
K122 Ubiquitination
K137 Ubiquitination
K141 Acetylation
K141 Ubiquitination
Y172 Phosphorylation
K173 Ubiquitination
S181 Phosphorylation P17612 (PRKACA) , Q13237 (PRKG2) , Q13464 (ROCK1)
T196 Phosphorylation
S199 Phosphorylation
S211 Phosphorylation
S214 Phosphorylation
S223 Phosphorylation
T236 Phosphorylation
T239 Phosphorylation
T240 Phosphorylation
K249 Ubiquitination
K253 Acetylation
K398 Acetylation
S414 Phosphorylation
Y442 Phosphorylation

研究背景

功能:

Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development. Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (By similarity).

翻译修饰:

Ubiquitinated. Ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (via C-terminus) with ZNF219. Interacts with DDRGK1.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

文献引用

1). Plasma Membrane-Derived Biomimetic Apoptotic Nanovesicles Targeting Inflammation and Cartilage Degeneration for Osteoarthritis. Small methods, 2024 (PubMed: 39036830) [IF=12.4]
2). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=7.5]

Application: WB    Species: Rat    Sample:

Fig. 8. GPX4 inhibitor RSL3 substantially attenuates VK2 protection of chondrocytes. (A-I) WB results for GPX4, NFκB, pMAPK/MAPK, Aggrecan, CollagenⅡ, SOX9, MMP3, MMP13 and Tubulin; (J) Quantitive analysis of relative Glutathione Peroxidase Activity; (K-L) GSH contents and ratio of GSH/GSSG; (M) Quantitative results of MDA content assay.
3). A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy. Stem Cell Research & Therapy, 2020 (PubMed: 32085810) [IF=7.5]

Application: IF/ICC    Species: Human    Sample: iPSCs

Fig. 7 The chondrogenic differentiation of iPSCs with different Li+ ions concentration in 14 days. a Immunofluorescence of chondrocytes specified proteins (COL II, Aggrecan, and SOX9) and hypertrophic specified protein (COL X and MMP13). b The average fluorescence intensity of COL II, Aggrecan, SOX9, MMP13, and COL X proteins in each group, n = 5. c The relative genes expression of chondrogenic differentiation cultured with Li+ ions, n = 3. d The relative genes expression of hypertrophic differentiation cultured with Li+ ions, n = 3. Data presented as mean ± SEM. Scale bar = 200 μm. CTR: MCDM without any Li+ ions. *p < 0.05, **p < 0.01, ***p < 0.001
4). Asiatic acid attenuates hypertrophic and fibrotic differentiation of articular chondrocytes via AMPK/PI3K/AKT signaling pathway. ARTHRITIS RESEARCH & THERAPY, 2020 (PubMed: 32398124) [IF=4.9]
5). Hypoxia-inducible factor prolyl hydroxylase inhibitor alleviates heatstroke-induced acute kidney injury by activating BNIP3-mediated mitophagy. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024 (PubMed: 38865198) [IF=4.8]
6). Region-specific effects of blocking estrogen receptors on longitudinal bone growth. JOURNAL OF ENDOCRINOLOGY, 2021 (PubMed: 34014834) [IF=4.0]
7). Ddit3 suppresses the differentiation of mouse chondroprogenitor cells. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2016 (PubMed: 27845261) [IF=4.0]

Application: WB    Species: mouse    Sample:

Fig. 5. Effects of C/EBP and Sox9 on the differentiation of ATDC5. (a and d) Relative mRNA levels of Sox9 and C/EBP in three groups. (b and c) Detection and quantification of Sox9 by Western blot and image J. (e and f) Detection and quantification of C/EBP by Western blot and image J. All data were expressed as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.0001, compared to scrambled group. t
8). Fatty acid binding protein 3 deficiency limits atherosclerosis development via macrophage foam cell formation inhibition. EXPERIMENTAL CELL RESEARCH, 2021 (PubMed: 34370993) [IF=3.7]

Application: WB    Species: Mice    Sample: macrophages

Fig. 3. FABP3 knockdown reduced macrophage foam cell formation. Murine peritoneal macrophages were maintained in the special culture medium at 37 ◦C in a 5 % CO2 atmosphere. The cells were infected with lentivirus carrying shFABP3 or shRNA NC for 24 h and subsequently exposed with ox-LDL (50 μg/ml) for 24 h. (A) Representative Oil Red O-stained images of macrophages and quantification of the staining. (B) Immunoblots of SRA1, CD36, SRB1, ABCA1, and ABCG1 in macrophages. (C) Quantification of relative mRNA expression of SRA1, CD36, SRB1, ABCA1, and ABCG1. **p < 0.01 denote significant differences between the Control and the ox-LDL groups, &&p < 0.01 denote significant differences between the ox-LDL shRNA NC and ox-LDL + shFABP3 groups.
9). Impaired proliferation of growth plate chondrocytes in a model of osteogenesis imperfecta. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022 (PubMed: 35561582) [IF=3.1]
10). Effect of tissue expansion on chondrocyte sheets in cartilage composite reconstruction. American Journal of Translational Research, 2023 (PubMed: 35035686) [IF=2.2]

Application: WB    Species: Rat    Sample:

Figure 8 Evaluation of protein expression and cartilage-related genes in the chondrocyte sheets cultured in vitro under static pressure and hypoxic conditions. (A) Protein expression of Aggrecan, COL II, Sox-9, and HIF-1α as determined using western blotting; β-actin was used as a loading control. The expression of Aggrecan (B), COL II (C), Sox-9 (D) and HIF1-α (E) genes was determined using real-time PCR. GAPDH was used as a housekeeping gene. Data are presented as the mean ± standard deviation (n=3), *P
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