(1)
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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6341, RRID:AB_2835197.
展开/折叠
Bmx; BMX non receptor tyrosine kinase; BMX_HUMAN; BONE MARROW KINASE, X-LINKED; Bone marrow tyrosine kinase gene in chromosome X protein; Cytoplasmic tyrosine protein kinase BMX; Cytoplasmic tyrosine-protein kinase BMX; Epithelial and endothelial tyrosine kinase; ETK; NKT38; NTK 38; NTK38; Protein tyrosine kinase BMX; PSCTK 2; PSCTK 3; PSCTK2; PSCTK3;
抗原和靶标
Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.
- P51813 BMX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51813 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y40 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| K172 | Acetylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Q08881 (ITK) , Q06187 (BTK) , P51813 (BMX) , P42680 (TEC) | Uniprot |
| Y224 | Phosphorylation | Q08881 (ITK) , P42680 (TEC) , P51813 (BMX) , Q06187 (BTK) | Uniprot |
| S226 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| Y330 | Phosphorylation | Uniprot | |
| K337 | Acetylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K347 | Acetylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| K395 | Acetylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| Y566 | Phosphorylation | P12931 (SRC) , P51813 (BMX) | Uniprot |
| S568 | Phosphorylation | Uniprot |
翻译修饰 - P51813 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P08581 (MET) | Y1235 | Uniprot |
| P11362 (FGFR1) | Y653 | Uniprot |
| P11362 (FGFR1) | Y654 | Uniprot |
| P51813 (BMX) | Y216 | Uniprot |
| P51813 (BMX) | Y224 | Uniprot |
| P51813 (BMX) | Y566 | Uniprot |
| P56945 (BCAR1) | Y12 | Uniprot |
| Q05397 (PTK2) | Y194 | Uniprot |
| Q05397 (PTK2) | Y441 | Uniprot |
| Q05397 (PTK2) | Y576 | Uniprot |
| Q05397 (PTK2) | Y577 | Uniprot |
| Q05397 (PTK2) | Y742 | Uniprot |
| Q05397 (PTK2) | Y861 | Uniprot |
| Q05397 (PTK2) | Y898 | Uniprot |
| Q05397 (PTK2) | Y925 | Uniprot |
| Q05397 (PTK2) | Y1007 | Uniprot |
| Q07912 (TNK2) | Y859 | Uniprot |
| Q07912 (TNK2) | Y860 | Uniprot |
| Q16611 (BAK1) | Y108 | Uniprot |
| Q96T51 (RUFY1) | Y389 | Uniprot |
| Q96T51 (RUFY1) | Y400 | Uniprot |
研究背景
Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.
Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-566 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.
Cytoplasm.
Note: Localizes to the edges of spreading cells when complexed with BCAR1.
Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.
Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.
SH2 domain mediates interaction with RUFY1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
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