NF kappaB p100/p52 Antibody - #AF6373

Fig. 6: Knockdown of FUT8 in OS cells in vitro activates TNF/NF-κB2 signaling pathway. A Western blot of proteins in a NF-κB2 signaling pathway show that this pathway was activated in MNNG-siF8 cells but inhibited in MNNG-F8 cells. B Analysis of p52 translocation in OS cell lines. Western blot probed for both in nuclear p52 and cytoplasmic p52. C Quantitation of ratio of nuclear p52 to cytoplasmic p52 expression. Mean ± SD (N = 3) ratio expression in MNNG-siF8 cells was higher, compared to controls, and lower in MNNG-F8 cells. Relative expression of nuclear p52 was normalized to PCNA while relative expression of cytoplasmic p52 was normalized to β-tubulin. D Representative p52 immunofluorescent images of cultured OS cell lines showed translocation of p52 into the nucleus. Red fluorescence represented nuclei, green fluorescence represented p52 (Scale bar: 25 μm). The translocation of p52 into the nuclei appeared visually to be enhanced in MNNG-siF8 cells but reduced in MNNG-F8 cells compared to control cells. E Quantitation of ratio of nuclear p52 to cytoplasmic p52 in cultured OS cell lines. Mean ± SD (N = 3) ratio of nuclear p52 fluorescence to cytoplasmic p52 fluorescence. *P 

Fig. 6: Knockdown of FUT8 in OS cells in vitro activates TNF/NF-κB2 signaling pathway. A Western blot of proteins in a NF-κB2 signaling pathway show that this pathway was activated in MNNG-siF8 cells but inhibited in MNNG-F8 cells. B Analysis of p52 translocation in OS cell lines. Western blot probed for both in nuclear p52 and cytoplasmic p52. C Quantitation of ratio of nuclear p52 to cytoplasmic p52 expression. Mean ± SD (N = 3) ratio expression in MNNG-siF8 cells was higher, compared to controls, and lower in MNNG-F8 cells. Relative expression of nuclear p52 was normalized to PCNA while relative expression of cytoplasmic p52 was normalized to β-tubulin. D Representative p52 immunofluorescent images of cultured OS cell lines showed translocation of p52 into the nucleus. Red fluorescence represented nuclei, green fluorescence represented p52 (Scale bar: 25 μm). The translocation of p52 into the nuclei appeared visually to be enhanced in MNNG-siF8 cells but reduced in MNNG-F8 cells compared to control cells. E Quantitation of ratio of nuclear p52 to cytoplasmic p52 in cultured OS cell lines. Mean ± SD (N = 3) ratio of nuclear p52 fluorescence to cytoplasmic p52 fluorescence. *P 

Figure 6. |The expression of components in the NF‑κB pathway in platelet‑derived growth factor‑BB‑activated HSC‑T6 treated with ASIV. (A‑E) The relative mRNA expression levels of (A) p65, (B) p52, (C) p50, (D) IKKα and (E) IκBα. (F) Protein expression of p65, p52 and IκBα. *P<0.05 and **P<0.01. IKKα, inhibitor of nuclear factor‑κB kinase subunit‑α; IκBα, inhibitor of nuclear factor‑κB‑α; NF‑κB, nuclear factor‑κB; ASIV, astragaloside IV.