产品: RelB 抗体
货号: AF6380
描述: Rabbit polyclonal antibody to RelB
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 70kDa; 62kD(Calculated).
蛋白号: Q01201
RRID: AB_2835221

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
RelB Antibody detects endogenous levels of total RelB.
RRID:
AB_2835221
引用格式: Affinity Biosciences Cat# AF6380, RRID:AB_2835221.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

I REL; I-Rel; IREL; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; relB; RELB_HUMAN; Reticuloendotheliosis viral oncogene homolog B; Transcription factor Rel B; Transcription factor RelB; v rel avian reticuloendotheliosis viral oncogene homolog B; v rel reticuloendotheliosis viral oncogene homolog B;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
RelB a transcription factor of the nuclear factor-kappaB (NFkB) transcription complex. Stimulates promoter activity in the presence NFkB1/NFkB-p50. Plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes.
序列:
MLRSGPASGPSVPTGRAMPSRRVARPPAAPELGALGSPDLSSLSLAVSRSTDELEIIDEYIKENGFGLDGGQPGPGEGLPRLVSRGAASLSTVTLGPVAPPATPPPWGCPLGRLVSPAPGPGPQPHLVITEQPKQRGMRFRYECEGRSAGSILGESSTEASKTLPAIELRDCGGLREVEVTACLVWKDWPHRVHPHSLVGKDCTDGICRVRLRPHVSPRHSFNNLGIQCVRKKEIEAAIERKIQLGIDPYNAGSLKNHQEVDMNVVRICFQASYRDQQGQMRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFAYDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPNHYREAAFGGGLLSPGPEAT

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
90
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q01201 作为底物

Site PTM Type Enzyme
R16 Methylation
S37 Phosphorylation
S50 Phosphorylation
T103 Phosphorylation
S116 Phosphorylation
S217 Phosphorylation
S221 Phosphorylation
K242 Ubiquitination
S254 Phosphorylation
Y293 Phosphorylation
K327 Ubiquitination
S425 Phosphorylation
S472 Phosphorylation O14920 (IKBKB) , O15111 (CHUK)
S573 Phosphorylation P49841 (GSK3B)
T579 Phosphorylation

研究背景

功能:

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function.

翻译修饰:

Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.

细胞定位:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with NEK6 in the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID. Interacts with ARNTL/BMAL1 and the interaction is enhanced in the presence of CLOCK (By similarity).

蛋白家族:

Both N- and C-terminal domains are required for transcriptional activation.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). Deficient DNASE1L3 facilitates neutrophil extracellular traps-induced invasion via cyclic GMP-AMP synthase and the non-canonical NF-κB pathway in diabetic hepatocellular carcinoma. Clinical & Translational Immunology, 2022 (PubMed: 35474906) [IF=5.8]

Application: WB    Species: Human    Sample: HCC cell

Figure 7 Roles of the non‐canonical NF‐κB pathway in HCC cell invasion. (a–d) Gene expression of NF‐κB pathway subunits NFKB2 (a) and RELB (c) in various HCC stages and adjacent normal tissues (Nor). Influence of these genes on the survival of HCC patients in the TCGA_LIHC cohort (b, d). (e, f) NETs DNA‐induced nuclear translocation of RelB in HCC SMMC7721 cells was observed with immunofluorescence imaging under a confocal microscope (n = 4). (g) RNA interference was applied to knock down RELB expression in SMMC7721 cells (n = 3). (h) NETs DNA‐induced invasion was observed between RELB‐downregulated and control SMMC7721 cells (n = 4). Data are shown as mean ± SD.

Application: IF/ICC    Species: Human    Sample: HCC cell

Figure 7 Roles of the non‐canonical NF‐κB pathway in HCC cell invasion. (a–d) Gene expression of NF‐κB pathway subunits NFKB2 (a) and RELB (c) in various HCC stages and adjacent normal tissues (Nor). Influence of these genes on the survival of HCC patients in the TCGA_LIHC cohort (b, d). (e, f) NETs DNA‐induced nuclear translocation of RelB in HCC SMMC7721 cells was observed with immunofluorescence imaging under a confocal microscope (n = 4). (g) RNA interference was applied to knock down RELB expression in SMMC7721 cells (n = 3). (h) NETs DNA‐induced invasion was observed between RELB‐downregulated and control SMMC7721 cells (n = 4). Data are shown as mean ± SD.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.