产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
抗原和靶标
A synthesized peptide derived from human PI4KB.
Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
- Q9UBF8 PI4KB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM
翻译修饰 - Q9UBF8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Acetylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S268 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Q15208 (STK38) | Uniprot |
| T280 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K290 | Methylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Q15139 (PRKD1) , Q9BZL6 (PRKD2) | Uniprot |
| S306 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| T426 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| T517 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| K534 | Ubiquitination | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| Y805 | Phosphorylation | Uniprot | |
| Y810 | Phosphorylation | Uniprot |
研究背景
Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
(Microbial infection) Plays an essential role in Aichi virus RNA replication. Recruited by ACBD3 at the viral replication sites.
Endomembrane system. Mitochondrion outer membrane>Peripheral membrane protein. Rough endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus. Golgi apparatus membrane. Cytoplasm>Perinuclear region.
Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3 (PubMed:24672044, PubMed:27009356, PubMed:28289207). GGA2 is also involved in the recruitment (PubMed:28289207).
Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6. Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3. Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN. Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane.
(Microbial infection) Interacts with Aichi virus protein 3A. Part of a complex Aichi virus protein 3A/ACBD3/PI4KB that allows the synthesis of PI4P at the viral RNA replication sites.
Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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