产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Protein RUFY3; rap2 interacting protein x; Rap2 interacting protein x isoform 2; Rap2-interacting protein x; RIPx; Rpipx; Rufy3; RUFY3_HUMAN; RUN and FYVE domain containing 3; Singar; Singar1; Single axon regulated protein; single axon related 1; Single axon-regulated protein;
抗原和靶标
A synthesized peptide derived from human RUFY3.
Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
- Q7L099 RUFY3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSALTPPTDMPTPTTDKITQAAMETIYLCKFRVSMDGEWLCLRELDDISLTPDPEPTHEDPNYLMANERMNLMNMAKLSIKGLIESALNLGRTLDSDYAPLQQFFVVMEHCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSMYLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELAVANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVEKELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH
研究背景
Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner.
Phosphorylated by PAK1. Isoform 1 is partially phosphorylated (By similarity).
Cytoplasm. Endomembrane system. Cell projection>Invadopodium. Perikaryon. Cell projection. Cell projection>Growth cone. Cell projection>Filopodium. Cell projection>Lamellipodium.
Note: Colocalizes with PAK1, F-actin, myosins and integrins in invadopodia at the cell periphery (PubMed:25766321). Colocalized with Ras-related Rab-5 proteins in cytoplasmic vesicles (PubMed:20376209). Accumulates in axon growth cones in a F-actin-dependent manner (By similarity). Colocalized with FSCN1 and F-actin at filipodia and lamellipodia of axonal growth cones (By similarity). Colocalized with DBN1 and F-actin at transitional domain of the axonal growth cone (By similarity).
Overexpressed in gastric cancer cells and tissues (at protein level).
Interacts with PAK1. Interacts (via C-terminus) with Ras-related Rab-5 proteins. Interacts (via C-terminus) with Ras-related Rap-2 proteins. Interacts with PIK3CA and PIK3R1 (By similarity). Interacts (via N-terminus) with FSCN1; this interaction induces neuron axon development (By similarity). Interacts with DBN1 (By similarity).
限制条款
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