Smad1 Antibody - #AF6451

Fig. 1. Inokosterone (IS) promoted cell viability and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). (A) Morphological observation of primary cultured BMSCs at 1, 3, 5, and 7 days ( × 100, Scale bar = 100 μm). Then, BMSCs were cultured in the osteogenic induction and differentiation medium with/without IS (50, 100, and 200 mg/L) for 72 h. (B) IS (50, 100, and 200 mg/L) promoted cell viability of BMSCs, which was measured by the cell counting kit-8 analysis (n = 5). (C) IS advanced the levels of collagen type I (collagen I), alkaline phosphatase (ALP), and osteocalcin (OCN) of cultured supernatant, which was detected by the enzyme-linked immunosorbent assay (n = 6). (D) Alizarin red S (ARS) staining showed the area of ARS staining of BMSC with IS treatment was increased (n = 3). (E) They are characteristic photos of alizarin red S staining ( × 400, Scale bar = 300 μm). The expression levels of (F) bone morphogenetic protein-2 (BMP2), (G) smad1, (H) runt-related transcription factor 2 (RUNX2), (I) collagen I, (J) ALP in BMSCs were measured by Western blot (n = 3). IS promoted their expression. (K) The characteristic photos of Western blot. The experiment was repeated thrice, and all data were expressed with mean ± standard deviation. *P < 0.05, **P < 0.01, compared to the control (CN) group.