产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6460, RRID:AB_2835280.
展开/折叠
Brain Ca(2+) calmodulin dependent protein kinase type 4; Brain Ca(2+) calmodulin dependent protein kinase type IV; Brain Ca++-calmodulin dependent protein kinase type IV; Calcium / calmodulin dependent protein kinase type 4 catalytic chain; Calcium / calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV; Calcium/calmodulin dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CAM kinase-GR; CaMK 4; CAMK GR; CaMK IV; Camk4; CaMKGR; IV; KCC4_HUMAN; MGC36771;
抗原和靶标
Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
- Q16566 KCC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16566 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Q8N5S9 (CAMKK1) , Q16566 (CAMK4) | Uniprot |
| S13 | Phosphorylation | Q8N5S9 (CAMKK1) , Q16566 (CAMK4) | Uniprot |
| Y32 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T57 | O-Glycosylation | Uniprot | |
| S58 | O-Glycosylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S137 | O-Glycosylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S189 | O-Glycosylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| T200 | Phosphorylation | Q8N5S9 (CAMKK1) , Q96RR4 (CAMKK2) | Uniprot |
| K279 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Q16566 (CAMK4) | Uniprot |
| S343 | Phosphorylation | Uniprot | |
| S344 | O-Glycosylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S345 | O-Glycosylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| S356 | O-Glycosylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Q16566 (CAMK4) | Uniprot |
| K404 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot |
翻译修饰 - Q16566 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P14866 (HNRNPL) | S544 | Uniprot |
| P16220 (CREB1) | S133 | Uniprot |
| P16949 (STMN1) | S16 | Uniprot |
| P29475-1 (NOS1) | S852 | Uniprot |
| P53667 (LIMK1) | T508 | Uniprot |
| P56524-1 (HDAC4) | S467 | Uniprot |
| P56524-1 (HDAC4) | S632 | Uniprot |
| Q04206 (RELA) | S536 | Uniprot |
| Q16566 (CAMK4) | S12 | Uniprot |
| Q16566 (CAMK4) | S13 | Uniprot |
| Q16566 (CAMK4) | S341 | Uniprot |
| Q16566 (CAMK4) | S360 | Uniprot |
| Q92793-1 (CREBBP) | S302 | Uniprot |
| Q99623 (PHB2) | S91 | Uniprot |
| Q9UQL6 (HDAC5) | S259 | Uniprot |
| Q9UQL6 (HDAC5) | S498 | Uniprot |
| Q9UQM7-2 (CAMK2A) | S332 | Uniprot |
研究背景
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13.
Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.
Cytoplasm. Nucleus.
Note: Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity).
Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
Monomer (By similarity). Interacts with protein phosphatase 2A (PPP2CA/PPP2CB); the interaction is mutually exclusive with binding to Ca(2+)/calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
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