XRCC1 Antibody - #AF0296

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产品: XRCC1 抗体
货号: AF0296
描述: Rabbit polyclonal antibody to XRCC1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Horse, Sheep, Rabbit, Dog
分子量: 90kDa; 69kD(Calculated).
蛋白号: P18887
RRID: AB_2833462

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Horse(80%), Sheep(88%), Rabbit(80%), Dog(100%)
克隆:
Polyclonal
特异性:
XRCC1 Antibody detects endogenous levels of total XRCC1.
RRID:
AB_2833462
引用格式: Affinity Biosciences Cat# AF0296, RRID:AB_2833462.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair complementing defective repair in chinese hamster cells 1; X ray repair cross complementing 1; X ray repair cross complementing protein 1; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P18887(XRCC1_HUMAN) >>Visit HPA database.

基因/基因ID:
XRCC1(7515)
表达:
P18887 XRCC1_HUMAN:

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

描述:
XRCC1 Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3).
序列:
MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSRRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDNMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
100
Sheep
88
Horse
80
Rabbit
80
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18887 作为底物

Site PTM Type Enzyme
K129 Ubiquitination
K138 Ubiquitination
S140 Phosphorylation
S151 Phosphorylation
S162 Phosphorylation
K169 Acetylation
K169 Ubiquitination
K176 Sumoylation
S184 Phosphorylation O96017 (CHEK2)
T198 Phosphorylation
S199 Phosphorylation
T202 Phosphorylation
S204 Phosphorylation
S210 Phosphorylation O96017 (CHEK2)
Y211 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation P24941 (CDK2)
S229 Phosphorylation P24941 (CDK2)
S234 Phosphorylation
T235 Phosphorylation
S236 Phosphorylation
S241 Phosphorylation
K247 Sumoylation
K256 Acetylation
K256 Sumoylation
T257 Phosphorylation
S259 Phosphorylation
K260 Acetylation
S266 Phosphorylation
S268 Phosphorylation
K271 Acetylation
T279 Phosphorylation
T281 Phosphorylation
T284 Phosphorylation O96017 (CHEK2)
T296 O-Glycosylation
T296 Phosphorylation
K298 Acetylation
R310 Methylation
K341 Ubiquitination
K348 Ubiquitination
S357 Phosphorylation
T358 Phosphorylation
T367 Phosphorylation
Y370 Phosphorylation
S371 Phosphorylation P78527 (PRKDC)
K383 Ubiquitination
S408 Phosphorylation P68400 (CSNK2A1)
S409 Phosphorylation P68400 (CSNK2A1)
S410 Phosphorylation P68400 (CSNK2A1)
S416 Phosphorylation P68400 (CSNK2A1)
S418 Phosphorylation P68400 (CSNK2A1)
S421 Phosphorylation P68400 (CSNK2A1)
K431 Ubiquitination
T437 Phosphorylation
T440 O-Glycosylation
T440 Phosphorylation
S446 O-Glycosylation
S446 Phosphorylation
S447 O-Glycosylation
S447 Phosphorylation
T453 O-Glycosylation
T453 Phosphorylation
T457 Phosphorylation
S461 Phosphorylation P68400 (CSNK2A1)
S475 Phosphorylation P68400 (CSNK2A1)
S485 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T488 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
Y515 Phosphorylation P68400 (CSNK2A1)
S518 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T519 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T523 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S525 Phosphorylation P68400 (CSNK2A1)
S578 Phosphorylation P68400 (CSNK2A1)
K620 Ubiquitination
Y627 Phosphorylation

研究背景

功能:

Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.

翻译修饰:

Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.

Sumoylated.

细胞定位:

Nucleus.
Note: Moves from the nucleoli to the global nuclear chromatin upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

亚基结构:

Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.

研究领域

· Genetic Information Processing > Replication and repair > Base excision repair.

文献引用

1). iTRAQ based proteomic analysis of PM2.5 induced lung damage. RSC Advances, 2019 (PubMed: 35517034) [IF=3.9]

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