产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6468, RRID:AB_2835287.
展开/折叠
AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740;
抗原和靶标
- Q8WXE1 ATRIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKPHSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGFGSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFLPLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8WXE1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Q13535 (ATR) | Uniprot |
| S72 | Phosphorylation | Q13535 (ATR) | Uniprot |
| K96 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S239 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K270 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| Y636 | Phosphorylation | Uniprot |
研究背景
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Phosphorylated by ATR.
Nucleus.
Note: Redistributes to discrete nuclear foci upon DNA damage.
Ubiquitous.
Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Belongs to the ATRIP family.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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