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Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries.

产品:	CPI17alpha 抗体
货号:	AF6473
描述:	Rabbit polyclonal antibody to CPI17alpha
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Sheep, Rabbit
分子量:	22kDa; 17kD(Calculated).
蛋白号:	Q96A00
RRID:	AB_2835292

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)

克隆:

Polyclonal

特异性:

CPI17alpha Antibody detects endogenous levels of total CPI17alpha.

RRID:

AB_2835292
引用格式: Affinity Biosciences Cat# AF6473, RRID:AB_2835292.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;

抗原和靶标

免疫原:

Uniprot:

Q96A00(PP14A_HUMAN) >>Visit HPA database.

基因/基因ID:

PPP1R14A(94274)

表达:

Q96A00 PP14A_HUMAN:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

描述:

PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.

序列:

Q96A00 PP14A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Xenopus

Horse

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96A00 作为底物

Q96A00

Site	PTM Type	Enzyme	Source
R5	Methylation		Uniprot
S12	Phosphorylation	O43293 (DAPK3)	Uniprot
S16	Phosphorylation		Uniprot
R19	Methylation		Uniprot
R21	Methylation		Uniprot
S26	Phosphorylation		Uniprot
K32	Methylation		Uniprot
T38	Phosphorylation	Q05655 (PRKCD) , P17612 (PRKACA) , O75116 (ROCK2) , Q02156 (PRKCE) , O43293 (DAPK3) , Q15139 (PRKD1) , Q13976 (PRKG1) , P17252 (PRKCA) , Q13418 (ILK) , Q05513 (PRKCZ) , Q13153 (PAK1) , P98161 (PKD1)	Uniprot
K40	Methylation		Uniprot
S128	Phosphorylation		Uniprot
S130	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S134	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot

研究背景

Q96A00_PP14A_HUMAN

功能:

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

细胞定位:

Cytoplasm.

组织特异性:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

蛋白家族:

Belongs to the PP1 inhibitor family.

研究领域

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

文献引用

1). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB Species: Rat Sample:

Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-CPI-17 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-CPI-17 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 vs. control group. CPI-17: C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa; p-CPI-17: phosphorylated C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa.

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CPI17alpha Antibody - #AF6473

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