(11)
(11)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6477, RRID:AB_2835296.
展开/折叠
Apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; ASK1; M3K5; M3K5_HUMAN; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5;
抗原和靶标
- Q99683 M3K5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99683 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | P11309 (PIM1) , P51812 (RPS6KA3) , P31749 (AKT1) , P31751 (AKT2) | Uniprot |
| R89 | Methylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| T528 | Phosphorylation | Uniprot | |
| K535 | Sumoylation | Uniprot | |
| Y570 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| K709 | Ubiquitination | Uniprot | |
| Y718 | Phosphorylation | P23458 (JAK1) , O60674 (JAK2) | Uniprot |
| K730 | Ubiquitination | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| T813 | Phosphorylation | Q99683 (MAP3K5) | Uniprot |
| Y814 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot | |
| T825 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| T838 | Phosphorylation | Q14680 (MELK) , Q99683 (MAP3K5) , O95382 (MAP3K6) , Q15139 (PRKD1) | Uniprot |
| T842 | Phosphorylation | Q99683 (MAP3K5) | Uniprot |
| C869 | S-Nitrosylation | Uniprot | |
| K946 | Acetylation | Uniprot | |
| S958 | Phosphorylation | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | O15530 (PDPK1) , Q99683 (MAP3K5) | Uniprot |
| Y982 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | Uniprot | |
| S997 | Phosphorylation | Uniprot | |
| S1029 | Phosphorylation | Uniprot | |
| S1033 | Phosphorylation | Uniprot | |
| T1059 | Phosphorylation | Uniprot | |
| K1064 | Ubiquitination | Uniprot | |
| K1083 | Sumoylation | Uniprot | |
| T1109 | Phosphorylation | P51812 (RPS6KA3) | Uniprot |
| K1114 | Sumoylation | Uniprot | |
| S1240 | Phosphorylation | Uniprot | |
| T1326 | Phosphorylation | P51812 (RPS6KA3) | Uniprot |
翻译修饰 - Q99683 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15530 (PDPK1) | S394 | Uniprot |
| O15530 (PDPK1) | S398 | Uniprot |
| P38936 (CDKN1A) | S98 | Uniprot |
| P45379-11 (TNNT2) | T191 | Uniprot |
| P45379-6 (TNNT2) | T194 | Uniprot |
| P45379-11 (TNNT2) | S195 | Uniprot |
| P45379-6 (TNNT2) | S198 | Uniprot |
| P45379-10 (TNNT2) | T201 | Uniprot |
| P45379 (TNNT2) | T204 | Uniprot |
| P45379-10 (TNNT2) | S205 | Uniprot |
| P45379 (TNNT2) | S208 | Uniprot |
| P45983 (MAPK8) | T183 | Uniprot |
| P45983 (MAPK8) | Y185 | Uniprot |
| P45985 (MAP2K4) | T261 | Uniprot |
| P46734 (MAP2K3) | S218 | Uniprot |
| P52564 (MAP2K6) | S207 | Uniprot |
| Q16539 (MAPK14) | T180 | Uniprot |
| Q16539 (MAPK14) | Y182 | Uniprot |
| Q969S3 (ZNF622) | S314 | Uniprot |
| Q969S3 (ZNF622) | T318 | Uniprot |
| Q99683 (MAP3K5) | T813 | Uniprot |
| Q99683 (MAP3K5) | T838 | Uniprot |
| Q99683 (MAP3K5) | T842 | Uniprot |
| Q99683 (MAP3K5) | S966 | Uniprot |
| Q9UER7 (DAXX) | S176 | Uniprot |
| Q9UER7 (DAXX) | S184 | Uniprot |
| Q9Y3F4 (STRAP) | T175 | Uniprot |
| Q9Y3F4 (STRAP) | S179 | Uniprot |
研究背景
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5.
Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
Cytoplasm. Endoplasmic reticulum.
Note: Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Abundantly expressed in heart and pancreas.
Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis. Interacts with SOCS1 which recognizes phosphorylation of Tyr-718 and induces MAP3K5/ASK1 degradation in endothelial cells. Interacts with the 14-3-3 family proteins such as YWHAB, YWHAE, YWHAQ, YWHAH, YWHAZ and SFN. Interacts with ARRB2, BIRC2, DAB2IP, IGF1R, MAP3K6/ASK2, PGAM5, PIM1, PPP5C, SOCS1, STUB1, TRAF2, TRAF6 and TXN. Interacts with ERN1 in a TRAF2-dependent manner. Interacts with calcineurin subunit PPP3R1 and with PPM1L (By similarity). Interacts (via N-terminus) with RAF1 and this interaction inhibits the proapoptotic function of MAP3K5. Interacts with DAB2IP (via N-terminus C2 domain); the interaction occurs in a TNF-alpha-dependent manner. Interacts with DUSP13/DUSP13A; may positively regulate apoptosis. Interacts with DAXX. Interacts with RC3H2.
(Microbial infection) Interacts with HIV-1 Nef; this interaction inhibits MAP3K5 signaling.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
文献引用
Application: WB Species: Mouse Sample:
Application: WB Species: human Sample: HT-29 cells
Application: WB Species: mice Sample: colon tissues
Application: WB Species: mouse Sample: liver
Application: WB Species: human Sample: LX-2 cells
Application: WB Species: rat Sample: RA cells
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