| 产品: | XRCC4 抗体 |
| 货号: | AF0299 |
| 描述: | Rabbit polyclonal antibody to XRCC4 |
| 应用: | WB |
| 反应: | Human |
| 预测: | Rabbit, Dog |
| 分子量: | 40kDa; 38kD(Calculated). |
| 蛋白号: | Q13426 |
| RRID: | AB_2833464 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0299, RRID:AB_2833464.
展开/折叠
DNA double strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; SSMED; X ray repair complementing defective repair in Chinese hamster cells 4; X ray repair cross complementing 4; X ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC 4; XRCC4; XRCC4_HUMAN;
抗原和靶标
- Q13426 XRCC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13426 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K210 | Sumoylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| Y229 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T233 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S237 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T264 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S315 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T323 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S328 | Phosphorylation | P78527 (PRKDC) | Uniprot |
研究背景
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
Nucleus.
Widely expressed.
Homodimer and homotetramer in solution. The homodimer associates with LIG4. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts directly with PRKDC but not with the XRCC6/Ku70 and XRCC5/Ku86 dimer. Interacts with APTX and APLF.
Belongs to the XRCC4 family.
研究领域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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