产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6491, RRID:AB_2835302.
展开/折叠
Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;
抗原和靶标
- Q9UQL6 HDAC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UQL6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K35 | Sumoylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S259 | Phosphorylation | O43318 (MAP3K7) , Q05655 (PRKCD) , Q13555 (CAMK2G) , Q7KZI7 (MARK2) , Q9UQM7 (CAMK2A) , Q16566 (CAMK4) , P57059 (SIK1) , Q15139 (PRKD1) , Q13131 (PRKAA1) , Q14012 (CAMK1) , P54646 (PRKAA2) | Uniprot |
| S278 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S279 | Phosphorylation | Q9Y463 (DYRK1B) , P17612 (PRKACA) , Q00535 (CDK5) | Uniprot |
| T288 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S368 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| T496 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | P54646 (PRKAA2) , Q14012 (CAMK1) , Q16566 (CAMK4) , Q13555 (CAMK2G) , Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , P57059 (SIK1) , Q15139 (PRKD1) , O94806 (PRKD3) , Q13131 (PRKAA1) , Q9BZL6 (PRKD2) | Uniprot |
| S504 | Phosphorylation | Uniprot | |
| K533 | Acetylation | Uniprot | |
| K533 | Sumoylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| K605 | Sumoylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| Y776 | Phosphorylation | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S1071 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot |
研究背景
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.
Ubiquitous.
Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with BCL6, DDIT3/CHOP, GRK5, KDM5B and MYOCD. Interacts with EP300 in the presence of TFAP2C. Interacts with ANKRA2. Interacts with CUL7 (as part of the 3M complex); negatively regulated by ANKRA2. Interacts with ZBTB7B; the interaction allows the recruitment of HDAC4 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression (By similarity). Interacts with RARA.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Belongs to the histone deacetylase family. HD type 2 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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