Collagen I Antibody - #AF7001

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产品: Collagen I 抗体
货号: AF7001
描述: Rabbit polyclonal antibody to Collagen I
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Bovine
预测: Pig, Zebrafish, Bovine, Horse, Dog, Chicken
分子量: 130-200 kDa; 139kD,129kD(Calculated).
蛋白号: P02452 | P08123
RRID: AB_2835309

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Bovine
预测:
Pig(100%), Zebrafish(89%), Horse(100%), Dog(100%), Chicken(89%)
克隆:
Polyclonal
特异性:
Collagen I Antibody detects endogenous levels of total Collagen I.
RRID:
AB_2835309
引用格式: Affinity Biosciences Cat# AF7001, RRID:AB_2835309.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha 1 type I collagen; Alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; alpha1(I) procollagen; CO1A1_HUMAN; COL1A1; COL1A2; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; Collagen type I alpha 1; Collagen type I alpha 2; EDSC; OI1; OI2; OI3; OI4; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain; Type I procollagen;

抗原和靶标

免疫原:

A synthesized peptide derived from human Collagen I, corresponding to a region within N-terminal amino acids.

Uniprot:

P02452(CO1A1_HUMAN) >>Visit HPA database.

P08123(CO1A2_HUMAN) >>Visit HPA database.

基因/基因ID:
COL1A2(1278) | COL1A1(1277)
表达:
P02452 CO1A1_HUMAN:

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

P08123 CO1A2_HUMAN:

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

描述:
This gene encodes one of the chains for type I collagen, the fibrillar collagen found in most connective tissues. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for alpha-1 type I collagen since alpha-2 is less abundant.
序列:
MFSFVDLRLLLLLAATALLTHGQEEGQVEGQDEDIPPITCVQNGLRYHDRDVWKPEPCRICVCDNGKVLCDDVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGNDGATGAAGPPGPTGPAGPPGFPGAVGAKGEAGPQGPRGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPGGPPGPKGNSGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGSPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPPGPAGAPGDKGESGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGAKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGGKGPRGETGPAGRPGEVGPPGPPGPAGEKGSPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPTGPVGPVGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGESMTDGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPVCFL

MLSFVDTRTLLLLAVTLCLATCQSLQEETVRKGPAGDRGPRGERGPPGPPGRDGEDGPTGPPGPPGPPGPPGLGGNFAAQYDGKGVGLGPGPMGLMGPRGPPGAAGAPGPQGFQGPAGEPGEPGQTGPAGARGPAGPPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGENGTPGQTGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGEIGAVGNAGPAGPAGPRGEVGLPGLSGPVGPPGNPGANGLTGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATGARGLVGEPGPAGSKGESGNKGEPGSAGPQGPPGPSGEEGKRGPNGEAGSAGPPGPPGLRGSPGSRGLPGADGRAGVMGPPGSRGASGPAGVRGPNGDAGRPGEPGLMGPRGLPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAVGTAGPSGPSGLPGERGAAGIPGGKGEKGEPGLRGEIGNPGRDGARGAPGAVGAPGPAGATGDRGEAGAAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGAKGPKGENGVVGPTGPVGAAGPAGPNGPPGPAGSRGDGGPPGMTGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRTGEVGAVGPPGFAGEKGPSGEAGTAGPPGTPGPQGLLGAPGILGLPGSRGERGLPGVAGAVGEPGPLGIAGPPGARGPPGAVGSPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNIGPVGAAGAPGPHGPVGPAGKHGNRGETGPSGPVGPAGAVGPRGPSGPQGIRGDKGEPGEKGPRGLPGLKGHNGLQGLPGIAGHHGDQGAPGSVGPAGPRGPAGPSGPAGKDGRTGHPGTVGPAGIRGPQGHQGPAGPPGPPGPPGPPGVSGGGYDFGYDGDFYRADQPRSAPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTGETCIRAQPENIPAKNWYRSSKDKKHVWLGETINAGSQFEYNVEGVTSKEMATQLAFMRLLANYASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEWGKTIIEYKTNKPSRLPFLDIAPLDIGGADQEFFVDIGPVCFK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
89
Chicken
89
Xenopus
78
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02452/P08123 作为底物

Site PTM Type Enzyme
K198 Acetylation
T209 Phosphorylation
S251 Phosphorylation
S389 Phosphorylation
S401 Phosphorylation
S404 Phosphorylation
S710 Phosphorylation
T981 Phosphorylation
K1064 Acetylation
T1073 Phosphorylation
S1104 Phosphorylation
S1124 Phosphorylation
T1138 O-Glycosylation
T1138 Phosphorylation
S1141 Phosphorylation
T1148 Phosphorylation
S1155 Phosphorylation
S1295 Phosphorylation

研究背景

功能:

Type I collagen is a member of group I collagen (fibrillar forming collagen).

翻译修饰:

Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.

Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.

O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

亚基结构:

Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2 (By similarity). Interacts with TRAM2. Interacts with MFAP4 in a Ca (2+)-dependent manner (By similarity).

蛋白家族:

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity).

Belongs to the fibrillar collagen family.

功能:

Type I collagen is a member of group I collagen (fibrillar forming collagen).

翻译修饰:

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

亚基结构:

Trimers of one alpha 2(I) and two alpha 1(I) chains.

蛋白家族:

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

Belongs to the fibrillar collagen family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon–Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects. Advanced Materials (PubMed: 36524686) [IF=29.4]

Application: WB    Species: Mouse    Sample:

Figure 7 Inflammatory response and bone repair ability in MRSA-infected bony tissue in vivo. The fluorescent images of a) tumor necrosis factor-alpha (TNF-α), c) interleukin-10 (IL-10), e) Runt-related transcription factor 2 (Runx-2), g) Collagen type I (COL I) and the corresponding mean optical intensity of b) TNF-α, d) IL-10, f) Runx-2, and h) COL I in the MRSA-infected bony tissues after treated with different samples for 14 days. The higher mean optical intensity indicates the more expression of the related proteins. Scale bars: 20 µm. Data were taken from independent samples (n = 6). The error bars indicate mean ± standard deviation: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001; ns: not significant (p > 0.05). The statistical analysis was performed using one-way analysis of variance with the Tukey multiple-comparisons test. i) 3D reconstruction (top) and extracted µ-CT images of the corresponding sections (bottom, indicated by the light blue areas) of harvested bone tissues after treated with different samples for 14 days.
2). Realising highly efficient sonodynamic bactericidal capability through the phonon–electron coupling effect using two‐dimensional catalytic planar defects. Advanced Materials (PubMed: 36524686) [IF=29.4]
3). Osteoblastic and anti-osteoclastic activities of strontium-substituted silicocarnotite ceramics: In vitro and in vivo studies. Bioactive Materials (PubMed: 32280833) [IF=18.9]
4). Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution. Advanced Science (PubMed: 36965031) [IF=15.1]

Application: IHC    Species: Human    Sample:

Figure 3 Characterization of matrisome clusters during early IVD formation. A) Representative IHC images of ACAN (upper lane) and COL1A1 (lower lane) in developing human IVDs at the indicated developmental stages. Arrows indicate the increased ECM in the human NP region. Scale bar, 100 µm. B) FPKM expression of the indicated signature genes in developing human NC/NP tissues captured by LCM during early IVD formation. N of 9, 10, 11, and 13 weeks = 4; N of 12 weeks = 3. C) Heatmap showing pairwise Pearson correlations of expressed matrisome genes in human developing NC/NP cells. D) The number of expressed genes associated with six matrisome patterns in the indicated human (upper) and mouse (lower) NC/NP cell subclusters. E) Representation analysis of GO categories showing different functions for TBXT+/T+ (left), CTSK+/Ctsk+ (middle), and KRT15+/Krt15+ clusters (right). Asterisks indicate the GO categories only enriched in human NC/NP subclusters. F) Violin plots showing the expression levels of representative genes associated with six matrisome patterns in the indicated human (left) and mouse (right) NC/NP cell subclusters. G) Representative IHC images of CTSK in human axial skeleton sections at the indicated developmental stages. The red arrow indicates the ECM in the NP region at the NPL stage. Scale bar, 200 µm. H) Representative images of lineage tracing in the Ctsk‐Cre;mT/mG mouse IVD. Scale bar, 200 µm. I) Dot plot showing the mean expression of selected mechanosensitive ion channel genes among seven human NC/NP cell subpopulations. The dot size indicates the percentage of cells in subclusters with detected expression.
5). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH (PubMed: 32710621) [IF=14.9]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
6). LncRNA DACH1 protects against pulmonary fibrosis by binding to SRSF1 to suppress CTNNB1 accumulation. Acta Pharmaceutica Sinica B (PubMed: 36176913) [IF=14.5]

Application: WB    Species: Mouse    Sample:

Figure 1 Loss of LncDACH1 results in pulmonary fibrosis in mice. (A) Micro-CT shows that the shadow area of the lungs in LncDACH1-KO mice was significantly greater than that of WT; n = 4. (B) Forced vital capacity (FVC), forced expiratory flow (FEF), FEV0.1, FEV0.1/FVC, inspiratory capacity (IC) as well as Flow-volume loops were reduced in LncDACH1-KO mice as detected by a flexiVent system; n = 4. (C) qRT-PCR validated that mRNA expression of Fn1, Col1a1, Col3a1 and Acta2 was promoted by LncDACH1 deficiency; n = 6. (D) The protein levels of FN1 and Collagen I detected by Western blot assays; n = 6. (E) Hydroxyproline content in LncDACH1-KO mice compared to WT mice; n = 6. (F, G) H&E and Masson staining reveal more exacerbated lung tissue morphology and increased fibrotic areas in LncDACH1-KO mice compared with WT mice; bar = 50 μm; n = 3. (H, I) Collagen I and α-SMA were increased in LncDACH1-KO mice as shown by immunohistochemical analysis; scale bar = 50 μm; n = 3. (J, K) Collagen I and α-SMA were stained by immunofluorescence analysis; bar = 50 μm; n = 3. Data are presented as mean ± SEM; ∗P < 0.05, ∗∗P < 0.01; ns, no significance. Micro-CT, micro computed tomography.
7). Gold nanoparticles targeting the autophagy–lysosome system to combat the inflammation-compromised osteogenic potential of periodontal ligament stem cells: From mechanism to therapy. Biomaterials (PubMed: 36030103) [IF=14.0]
8). Fusion peptide engineered “statically-versatile” titanium implant simultaneously enhancing anti-infection, vascularization and osseointegration. Biomaterials (PubMed: 33069134) [IF=14.0]
9). Vascular Derived ECM Improves Therapeutic Index of BMP‐2 and Drives Vascularized Bone Regeneration. Small (PubMed: 35218305) [IF=13.3]
10). Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis. Autophagy (PubMed: 34964701) [IF=13.3]
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