Cox2 Antibody - #AF7003

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产品: Cox2 抗体
货号: AF7003
来源: Rabbit
应用: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 80kD, 70 kD; 69kD(Calculated).
蛋白号: P35354
RRID: AB_2835311

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   规格 价格 库存
 50ul ￥1250 现货
 100ul ￥2300 现货
 200ul ￥3000 现货

货期: 当天发货

相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
克隆:
Polyclonal
特异性:
Cox2 Antibody detects endogenous levels of total Cox2.
RRID:
AB_2835311
引用格式: Affinity Biosciences Cat# AF7003, RRID:AB_2835311.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase; Cyclooxygenase-2; Cyclooxygenase2; EC 1.14.99.1; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524; PES-2; PGG/HS; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2; ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10;

抗原和靶标

免疫原:
Uniprot:

P35354(PGH2_HUMAN) >>Visit HPA database.

基因/基因ID:
PTGS2(5743)
蛋白描述:
COX-2 Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
蛋白序列:
MLARALLLCAVLALSHTANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35354 作为底物

Site PTM Type Enzyme
N53 N-Glycosylation
Y120 Phosphorylation P07948 (LYN)
N130 N-Glycosylation
K155 Ubiquitination
K166 Ubiquitination
K346 Ubiquitination
N396 N-Glycosylation
K445 Ubiquitination
Y446 Phosphorylation P06241 (FYN)
N580 N-Glycosylation

研究背景

功能:

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia (By similarity).

翻译修饰:

S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.

细胞定位:

Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the prostaglandin G/H synthase family.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

文献引用

1). Fan X et al. Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation. Eur J Med Chem 2020 May 1;193:112217. (PubMed: 32182488) [IF=7.088]

Application: WB    Species: Mouse    Sample: BV2 and C6 cells

Fig. 2. Effects of compounds on protein expressions of iNOS, COX-2 in LPS-induced BV2 cell lines (A) and effects of compounds on protein expressions of iNOS in LPS-induced C6 cell lines (B). BV2 cell lines were exposed to the indicated compounds at concentrations of 10, 20 and 40 mM for 24 h in the presence of LPS (1 mg/mL). C6 cell lines were exposed to the indicated compounds at concentrations of 10, 20 and 40 mM for 24 h in the presence of LPS (10 mg/mL). The expression of iNOS and COX-2 was assayed and calculated by image J. Each error bar represents the mean ± SD of three independent experiments. #, p < 0.05 versus the control group; ##, p < 0.01 versus the control group; ###, p < 0.001 versus the control group. *, p < 0.05, **, p < 0.01 when compared to the LPS-treated cells. All data are the mean ± SD of at least three independent experiments.
2). Lu J et al. Polysaccharides From the Aerial Parts of Tetrastigma Hemsleyanum Diels et Gilg Induce Bidirectional Immunity and Ameliorate LPS-Induced Acute Respiratory Distress Syndrome in Mice. Front Pharmacol 2022 Mar 11;13:838873. (PubMed: 35370633) [IF=5.810]

Application: WB    Species: mouse    Sample: RAW264.7 cells

FIGURE 3 | Effects of SYQP on the release of inflammatory cytokines and Oxidative stress in LPS-induced RAW264.7 cells.(F–J) RAW264.7 cells were treated in previous mentioned ways, and the gene and protein expression of iNOS and COX-2 were examined.
3). Xian S et al. The Protective Effect of Evodiamine in Osteoarthritis: An In Vitro and In Vivo Study in Mice Model. Front Pharmacol 2022 Jun 20;13:899108. (PubMed: 35795554) [IF=5.810]

Application: WB    Species: Mouse    Sample: chondrocytes

FIGURE 3 Effects of evodiamine on IL-1β–induced inflammation in mouse chondrocytes. (A,B,C) Level of expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in chondrocytes of the three groups were evaluated by Western blot. The data were expressed as mean ± SD. Significant differences between different groups are indicated as ##p < 0.01, vs. control group; ∗∗ p < 0.01, vs. IL-1β alone stimulation group, n = 3.
4). Zhang YX et al. Antidepressant-like effects of helicid on a chronic unpredictable mild stress-induced depression rat model: Inhibiting the IKK/IκBα/NF-κB pathway through NCALD to reduce inflammation. Int Immunopharmacol 2021 Apr;93:107165. (PubMed: 33578182) [IF=5.714]

Application: WB    Species: Rat    Sample: hippocampus

Fig. 7. Changes of inflammatory factors in the hippocampus after AAV-siNCALD using western blotting. (A) Representative iNOS, COX-2, TNF-α, IL-1β, IL-6, and β-actin bands. (B), (C), (D), (E), (F) The relative ratios of grayscale values of iNOS, COX-2, TNF-α, IL-1β, and IL-6 to that of corresponding β-actin, n = 4. ###P < 0.001, CUMS + saline vs. sham; *P < 0.05; **P < 0.01; ***P < 0.001, CUMS + saline vs. CUMS + AAV-siNCALD. All data are shown as the mean ± SEM.
5). Zhang Y et al. 6′-O-Galloylpaeoniflorin attenuates Helicobacter pylori-associated gastritis via modulating Nrf2 pathway. Int Immunopharmacol 2022 Aug 11;111:109122. (PubMed: 35964411) [IF=5.714]
6). Jiao J et al. Mechanisms of pancreatic tumor suppression mediated by Xiang-lian pill: An integrated in silico exploration and experimental validation. J Ethnopharmacol 2022 Nov 15;298:115586. (PubMed: 35931303) [IF=5.195]
7). Cui Q et al. A network pharmacology approach to investigate the mechanism of Shuxuening injection in the treatment of ischemic stroke. J Ethnopharmacol 2020 Apr 18:112891 (PubMed: 32315738) [IF=5.195]

Application: WB    Species: rat    Sample: hippocampal

Fig. 13. |Expression of protein of three genes detected through Western blot. Note: Sham vs I/R, *P < 0.05; SXNI vs I/R, #P < 0.05.
8). Zhang H et al. Mst2 Overexpression Inhibits Thyroid Carcinoma Growth and Metastasis by Disrupting Mitochondrial Fitness and Endoplasmic Reticulum Homeostasis. J Oncol 2021 Sep 6;2021:1262291. (PubMed: 34557228) [IF=4.501]
9). Liu R et al. Ibuprofen Exerts Antiepileptic and Neuroprotective Effects in the Rat Model of Pentylenetetrazol-Induced Epilepsy via the COX-2/NLRP3/IL-18 Pathway. Neurochem Res 2020 Aug 13. (PubMed: 32789796) [IF=4.414]

Application: IHC    Species: Rat    Sample: brain tissue

Fig. 3 Immunohistochemistry showing the expression of COX-2 in the brain tissue of rats in each group. a Immunohistochemistry of COX-2 in the CA1 area of the hippocampus. Calibration bars: 50 μm. b The comparison of the mean optical density (MOD) of COX-2 in diferent groups (*P<0.05, **P<0.01; one-way ANOVA)

Application: WB    Species: Rat    Sample: brain tissue

Fig. 4 Western blot for COX-2 in the brain tissue of rats in each group (*P<0.05, **P<0.01; one-way ANOVA)
10). Hu Y et al. Coixol Suppresses NF-κB, MAPK Pathways and NLRP3 Inflammasome Activation in Lipopolysaccharide-Induced RAW 264.7 Cells. Molecules 2020 Feb 18;25(4) (PubMed: 32085388) [IF=4.411]

Application: WB    Species: Mice    Sample: RAW 264.7 cells

Figure 3 Effects of coixol on the expression of proinflammatory mediators in LPS-induced RAW 264.7 cells. Cells were pretreated with coixol for 4 h and then stimulated with 1μg/mL LPS for 4 h. (A) The production of nitric oxide (NO) in cells supernatant. NO was measured by Griess assay. The protein levels of (B) inducible-nitric oxide synthase (iNOS) and (C) cyclooxygenase (COX)-2. Protein levels were measured by western blotting. GAPDH was used as internal control. The data represent the mean ± SD (NO, n = 6; iNOS, COX-2, n = 3). * p < 0.05 vs. control group. # p < 0.05 vs. LPS group.
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