TIMP2 Antibody - #AF7008

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(3)   (10)  
产品: TIMP2 抗体
货号: AF7008
描述: Rabbit polyclonal antibody to TIMP2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 24kDa; 24kD(Calculated).
蛋白号: P16035
RRID: AB_2835316

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(85%), Horse(100%), Sheep(92%), Rabbit(85%), Dog(92%), Chicken(85%)
克隆:
Polyclonal
特异性:
TIMP2 Antibody detects endogenous levels of total TIMP2.
RRID:
AB_2835316
引用格式: Affinity Biosciences Cat# AF7008, RRID:AB_2835316.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CSC 21K; CSC-21K; CSC21K; Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2 precursor; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; TIMP2_HUMAN; Tissue Inhibitor of Metalloproteinase 2; Tissue inhibitor of metalloproteinases 2;

抗原和靶标

免疫原:
Uniprot:

P16035(TIMP2_HUMAN) >>Visit HPA database.

基因/基因ID:
TIMP2(7077)
描述:
TIMP2 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
序列:
MGAAARTLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
92
Dog
92
Bovine
85
Chicken
85
Rabbit
85
Xenopus
77
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P16035 作为底物

Site PTM Type Enzyme
K53 Ubiquitination
S57 Phosphorylation
Y62 Phosphorylation
K67 Ubiquitination
K74 Ubiquitination
Y90 Phosphorylation
Y165 Phosphorylation

研究背景

功能:

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

翻译修饰:

The activity of TIMP2 is dependent on the presence of disulfide bonds.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity.

蛋白家族:

Belongs to the protease inhibitor I35 (TIMP) family.

文献引用

1). Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 37240089) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 4 The effect of exposure of pregnant Wistar rat dams to fumonisins on COMP, VEGF, MMP-8, OPG, RANKL and TIMP-2 protein expression in female (a) and male (b) offspring femora. The protein levels are normalized to the corresponding β-actin levels and expressed as the fold change relative to the amount present in 0 FB control group. 0 FB—the control group; 60 FB—the group of offspring of pregnant rats receiving during pregnancy fumonisins at the dose of 60 FB/kg b.w.; 90 FB—the group of offspring of pregnant rats receiving during pregnancy fumonisins at the dose of 90 FB/kg b.w. Data are mean values ± SD (n = 3). Uncropped original Western blot membranes can be found in Supplementary Figure S1. Statistical significance
2). Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Experimental and Therapeutic Medicine, 2021 (PubMed: 33791004) [IF=2.7]

Application: IHC    Species: Rat    Sample: scleral tissue

Figure 2 Expression of lumican, MMP-2, MMP-14 and TIMP-2 in scleral tissue. Expression levels of lumican, MMP-2, MMP-14 and TIMP-2 expression at the (A) mRNA and (B) protein levels, detected by reverse transcription-quantitative PCR and western blotting, respectively. (C) Immunohistochemical analysis of lumican, MMP-2, MMP-14 and TIMP-2 expression. (D) Quantification of results presented in panel C. *P<0.05 vs. control eyes (paired t-tests). TIMP, tissue inhibitor of metalloproteinases.

Application: WB    Species: Rat    Sample: scleral tissue

Figure 5 MMP-2, MMP-14 and TIMP-2 expression in rat scleral fibroblasts following lumican overexpression or knockdown. *P<0.05 vs. the control group (one-way ANOVA and Tukey's post hoc tests). TIMP, tissue inhibitor of metalloproteinases.

Application: IHC    Species: rat    Sample: scleral

Figure 2. |Expression of lumican, MMP‑2, MMP‑14 and TIMP‑2 in scleral tissue. (C) Immunohistochemical analysis of lumican, MMP‑2, MMP‑14 and TIMP‑2 expression.
3). Comparison of MMP‐2 and TIMP‐2 expressions in yak testes at different ages. Reproduction in Domestic Animals, 2023 (PubMed: 36932642) [IF=1.7]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.