VEGFB Antibody - #AF7019

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产品: VEGFB 抗体
货号: AF7019
描述: Rabbit polyclonal antibody to VEGFB
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep
分子量: 17,21kDa; 22kD(Calculated).
蛋白号: P49765
RRID: AB_2835323

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(80%), Horse(90%), Sheep(80%)
克隆:
Polyclonal
特异性:
VEGFB Antibody detects endogenous levels of total VEGFB.
RRID:
AB_2835323
引用格式: Affinity Biosciences Cat# AF7019, RRID:AB_2835323.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Vascular endothelial growth factor B; Vascular endothelial growth factor-related factor; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF;

抗原和靶标

免疫原:
Uniprot:

P49765(VEGFB_HUMAN) >>Visit HPA database.

基因/基因ID:
VEGFB(7423)
表达:
P49765 VEGFB_HUMAN:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

描述:
Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins des- ignated VEGF-B and VEGF-C share a significant degree of homology withVEGF.
序列:
MSPLLRRLLLAALLQLAPAQAPVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
90
Pig
80
Bovine
80
Sheep
80
Dog
70
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

翻译修饰:

VEGF-B186 is O-glycosylated.

细胞定位:

Secreted.
Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

亚基结构:

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

蛋白家族:

Belongs to the PDGF/VEGF growth factor family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy. Molecular Medicine Reports, 2021 (PubMed: 33846768) [IF=3.4]

Application: WB    Species: Rat    Sample: kidney tissue

Figure 6. AS-IV activates the suppressed autophagy in the liver of T2DM rats. (A) Representative images of Beclin1, LC3 and P62 (immunohistochemical staining; magnification, ×400). Quantitative analysis of (B) Beclin1, (C) LC3 and (D) P62 were normalized to the normal control group. (E) Protein expression levels of Beclin1, LC3 and P62 in the liver tissues were detected via western blotting. Semi-quantitative analysis of (F) Beclin1, (G) LC3 and (H) P62 was normalised to β-actin. Data are presented as the mean ± SD, n=3. *P<0.05, ***P<0.001 vs. control group; #P<0.05, ##P<0.01, ###P<0.001 vs. model group. Con, Control; Mod, Model; AS-IV, Astragaloside IV (80 mg/kg); Met, Metformin (200 mg/kg).
2). Preventive and inhibitive effects of Yiwei Xiaoyu granules on the development and progression of spasmolytic polypeptide-expressing metaplasia lesions. World Journal of Gastrointestinal Oncology, 2021 (PubMed: 34853647) [IF=3.0]

Application: IHC    Species: Mice    Sample:

Figure 9 The expression of vascular endothelial growth factor-β was restored with Yiwei Xiaoyu granule administration in the spasmolytic polypeptide-expressing metaplasia lesions. A: The expression of vascular endothelial growth factor-β was upregulated with Yiwei Xiaoyu granules administration by immunohistochemistry measurement. B: Statistical differences existed between the control and the model (bP < 0.01). YWXY-H: High dose of Yiwei Xiaoyu Granules; YWXY-L: Low dose of Yiwei Xiaoyu Granules; VEGF: Vascular endothelial growth factor.

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