PDLIM1 Antibody - #AF0303

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产品: PDLIM1 抗体
货号: AF0303
描述: Rabbit polyclonal antibody to PDLIM1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa; 36kD(Calculated).
蛋白号: O00151
RRID: AB_2833467

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PDLIM1 Antibody detects endogenous levels of total PDLIM1.
RRID:
AB_2833467
引用格式: Affinity Biosciences Cat# AF0303, RRID:AB_2833467.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C terminal LIM domain protein 1; C-terminal LIM domain protein 1; Carboxy terminal LIM domain protein 1; CLIM 1; CLIM1; CLP 36; CLP36; Elfin; Enigma; Enigma homolog; hCLIM 1; hCLIM1; LIM domain protein CLP 36; LIM domain protein CLP-36; LIM domain protein CLP36; PDLI1_HUMAN; PDLIM 1; Pdlim1; PDZ and LIM domain 1; PDZ and LIM domain protein 1;

抗原和靶标

免疫原:
Uniprot:

O00151(PDLI1_HUMAN) >>Visit HPA database.

基因/基因ID:
PDLIM1(9124)
表达:
O00151 PDLI1_HUMAN:

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

描述:
CLIM1 a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
序列:
MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00151 作为底物

Site PTM Type Enzyme
T2 Acetylation
K22 Acetylation
K22 Ubiquitination
S31 Phosphorylation
T34 Phosphorylation
K71 Ubiquitination
C73 S-Nitrosylation
K87 Methylation
K87 Ubiquitination
S90 Phosphorylation
T94 O-Glycosylation
K98 Acetylation
K98 Ubiquitination
S108 Phosphorylation
S118 Phosphorylation
S123 Phosphorylation
T128 Phosphorylation
S130 Phosphorylation
S133 Phosphorylation
S134 Phosphorylation
T136 Phosphorylation
T141 Phosphorylation
Y144 Phosphorylation
Y151 Phosphorylation
S152 Phosphorylation
S153 Phosphorylation
S186 Phosphorylation
S187 Phosphorylation
K192 Ubiquitination
S194 Phosphorylation
Y197 Phosphorylation
K198 Ubiquitination
K203 Ubiquitination
K211 Ubiquitination
S213 Phosphorylation
T214 Phosphorylation
S215 Phosphorylation
K233 Ubiquitination
S235 Phosphorylation
S239 Phosphorylation
K241 Ubiquitination
K246 Acetylation
K246 Ubiquitination
S250 Phosphorylation
K256 Ubiquitination
T291 Phosphorylation
Y306 Phosphorylation
K309 Ubiquitination
T316 Phosphorylation
Y321 Phosphorylation
T325 Phosphorylation

研究背景

功能:

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

细胞定位:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Associates with actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

亚基结构:

Interacts with ACTN1, ACTN2 and ACTN4. Interacts with PDLIM4 (By similarity).

文献引用

1). O-GlcNAcylation of Keratin 18 coordinates TCA cycle to promote cholangiocarcinoma progression. bioRxiv, 2023

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