产品: HMGA2 抗体
货号: AF4069
描述: Rabbit polyclonal antibody to HMGA2
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Sheep
分子量: 18kd; 12kD(Calculated).
蛋白号: P52926
RRID: AB_2835351

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(80%), Bovine(100%), Sheep(100%)
克隆:
Polyclonal
特异性:
HMGA2 Antibody detects endogenous levels of total HMGA2.
RRID:
AB_2835351
引用格式: Affinity Biosciences Cat# AF4069, RRID:AB_2835351.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HMGA 2; BABL; High mobility group (nonhistone chromosomal) protein isoform I C; High mobility group (nonhistone chromosomal) protein isoform IC; High Mobility Group AT hook 2; High Mobility Group AT hook protein 2; High mobility group AT-hook protein 2; High mobility group protein HMGI C; High mobility group protein HMGI-C; High mobility group protein HMGIC; High mobility group protein I, isoform C; HMGA2; HMGA2_HUMAN; HMGI C; HMGIC; LIPO; Non histone chromosomal architectural protein HMGI C; Non histone chromosomal architectural protein HMGIC; Pygmy; STQTL9;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
序列:
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Pig
80
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52926 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
R4 Methylation
S12 Phosphorylation
T13 Phosphorylation
S14 Phosphorylation
K26 Acetylation
K26 Methylation
K26 Ubiquitination
T40 Phosphorylation
S44 Phosphorylation P06493 (CDK1)
S59 Phosphorylation P06493 (CDK1)
T97 Phosphorylation
T100 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S105 Phosphorylation

研究背景

功能:

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity).

翻译修饰:

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2 (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with E4F1. Interacts with NEK2 (By similarity).

蛋白家族:

Belongs to the HMGA family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis. Cancer Cell International, 2021 (PubMed: 34281530) [IF=5.8]

Application: WB    Species: Human    Sample: MDA-MB-453, MDA-MB-231 and MCF-10A cells

Fig. 5 The relationship of miR-216b and HMGA2. A The binding sequence of miR-216b and HMGA2. B, C Luciferase activity was examined in cells with co-transfection of HMGA2-WT or HMGA2-MUT and miR-216b mimic or miR-con. D HMGA2 abundance in cells with transfection of biotin-miR-con or biotin-miR-216b after RNA pull-down. E HMGA2 protein expression in MDA-MB-453, MDA-MB-231 and MCF-10A cells. F HMGA2 abundance in 5-FU-resistant and sensitive MDA-MB-231 and MDA-MB-453 cells. G, H HMGA2 protein level in cells with transfection of miR-con, miR-216b mimic, in-miR-con or in-miR-216b. Mock: non-transfected group. * P < 0.05

2). Long non-coding RNA LINC00460 promotes proliferation and inhibits apoptosis of cervical cancer cells by targeting microRNA-503-5p. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32740791) [IF=4.3]

Application: WB    Species: human    Sample: SiHa and HeLa cells

Fig. 4 | LINC00460 acts as a competing endogenous RNA of miR503-5p in SiHa and HeLa cells.d–h Expressions of AKT2,HMGA2 and SHOX2 in SiHa and HeLa cells by western blotting.**p<0.01 versus cells co-transfected with wild-type LINC00460 and miR-503-5p mimic. ##p<0.01 versus cells transfected with NC shRNA. WT wild-type LINC00460, MUT mutated LINC00460

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