ADAM 17 Antibody - #AF6361

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产品: ADAM 17 抗体
货号: AF6361
描述: Rabbit polyclonal antibody to ADAM 17
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Dog
分子量: 93 kDa; 93kD(Calculated).
蛋白号: P78536
RRID: AB_2835372

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
ADAM 17 Antibody detects endogenous levels of total ADAM 17.
RRID:
AB_2835372
引用格式: Affinity Biosciences Cat# AF6361, RRID:AB_2835372.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); A disintegrin and metalloproteinase domain 17; ADA17_HUMAN; ADAM 17; ADAM metallopeptidase domain 17; ADAM17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; Disintegrin and metalloproteinase domain-containing protein 17; MGC71942; NISBD; NISBD1; Snake venom like protease; Snake venom-like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme;

抗原和靶标

免疫原:
Uniprot:

P78536(ADA17_HUMAN) >>Visit HPA database.

基因/基因ID:
ADAM17(6868)
表达:
P78536 ADA17_HUMAN:

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

描述:
This gene encodes a disintegrin and metalloprotease (ADAM) domain 17, which is a member of the ADAM protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. This gene contains 19 exons and alternative splicing generates 2 transcripts.
序列:
MRQSLLFLTSVVPFVLAPRPPDDPGFGPHQRLEKLDSLLSDYDILSLSNIQQHSVRKRDLQTSTHVETLLTFSALKRHFKLYLTSSTERFSQNFKVVVVDGKNESEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVHRVKRRADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIEQIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYSPVGKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGNAEDDTVCLDLGKCKDGKCIPFCEREQQLESCACNETDNSCKVCCRDLSGRCVPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDKQYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDSHMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P78536 作为底物

Site PTM Type Enzyme
Y82 Phosphorylation
T84 Phosphorylation
T87 Phosphorylation
K102 Ubiquitination
K168 Ubiquitination
K182 Ubiquitination
K197 Ubiquitination
K302 Ubiquitination
K309 Ubiquitination
K376 Ubiquitination
Y379 Phosphorylation
S382 Phosphorylation
K388 Ubiquitination
K392 Ubiquitination
Y433 Phosphorylation
Y436 Phosphorylation
K460 Ubiquitination
K465 Ubiquitination
K620 Ubiquitination
S706 Phosphorylation
K728 Ubiquitination
T735 Phosphorylation P27361 (MAPK3) , P17252 (PRKCA) , Q16539 (MAPK14) , P28482 (MAPK1) , Q05655 (PRKCD) , Q02156 (PRKCE)
S747 Phosphorylation
K753 Ubiquitination
T761 Phosphorylation
S767 Phosphorylation
T768 Phosphorylation
S770 Phosphorylation
K779 Ubiquitination
S785 Phosphorylation
K790 Ubiquitination
S791 Phosphorylation
T796 Phosphorylation
K805 Ubiquitination
S808 Phosphorylation
K810 Ubiquitination
S819 Phosphorylation

研究背景

功能:

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity).

翻译修饰:

The precursor is cleaved by a furin endopeptidase.

Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

亚基结构:

Interacts with MAD2L1, MAPK14 and MUC1. Interacts with iRhom1/RHBDF1 and iRhom2/RHBDF2. Interacts with FRMD8 via its interaction with iRhom1/RHBDF1 and iRhom2/RHBDF2.

蛋白家族:

Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR (By similarity).

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

研究领域

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

文献引用

1). MicroRNA-145 overexpression attenuates apoptosis and increases matrix synthesis in nucleus pulposus cells. LIFE SCIENCES, 2019 (PubMed: 30797016) [IF=6.1]

Application: WB    Species: human    Sample: NP cells

Fig. 6. |ADAM17 suppression enhanced matrix metabolism in NP cells.(A,B) Western blotting (A) and subsequent densitometric analysis (B) revealed that collagen IIa and aggrecan protein expression in rat NP cells was increased by the ADAM17 inhibitor. GAPDH was used as the control. (C) Immunofluorescence imaging of human NP cells treated with LV-shControl and LV-shADAM17. (D, E)Western blotting (D) and subsequent densitometric analysis (E) demonstrated that ADAM17 suppression by LV-shADAM17 markedly increased collagen IIa and aggrecan protein expression in human NP cells.
2). GTS-21 attenuates ACE/ACE2 ratio and glycocalyx shedding in lipopolysaccharide-induced acute lung injury by targeting macrophage polarization derived ADAM-17. International immunopharmacology, 2024 (PubMed: 38310766) [IF=5.6]

Application: WB    Species: Mouse    Sample: lung tissue

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD.

Application: IF/ICC    Species: Mouse    Sample: lung tissue

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD.
3). Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling. Biological trace element research, 2020 (PubMed: 31797207) [IF=3.9]

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