WB, IHC, IF/ICC, ELISA

Hm, Ms, Rt, Zf, Pg, Bv, Rb, Dg, Mk, Fs

货号	产品	规格	来源	应用	反应	文献引用
AF4012	GSDMD Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	111
AF5418	Caspase 1 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	125
AF4005	Cleaved-Caspase 1 (Asp296), p20 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	102
AF4006	Cleaved-IL-1 beta (Asp116) Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Zf	81
AF5103	IL1 beta Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	281
AF5130	Caspase 4 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	5
DF7664	Caspase 5 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	1
AF7020	HMGB1 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	27
AF7018	beta Actin Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Pg,Zf,Bv,Rb,Dg,Mk,Fs	747
S0001	Goat Anti-Rabbit IgG HRP	100ul	Goat	WB,IHC,ELISA	Rb	528

Pyroptosis is a highly inflammatory form of programmed cell death that plays a critical role in host defense against microbial infections and in regulating inflammatory responses. It is characterized by rapid cell swelling, plasma membrane rupture, and release of pro-inflammatory intracellular contents, including cytokines and danger-associated molecular patterns (DAMPs), which trigger inflammation and recruit immune cells to the site of infection or tissue damage.

Store at -20 °C. Stable for 12 months from date of receipt.

Pyroptosis is a highly inflammatory form of programmed cell death that plays a critical role in host defense against microbial infections and in regulating inflammatory responses. It is characterized by rapid cell swelling, plasma membrane rupture, and release of pro-inflammatory intracellular contents, including cytokines and danger-associated molecular patterns (DAMPs), which trigger inflammation and recruit immune cells to the site of infection or tissue damage.

The pyroptotic pathway is primarily mediated by a family of cytosolic pattern recognition receptors (PRRs) known as inflammasomes. In response to microbial pathogens or cellular stress, inflammasomes assemble and activate caspase-1, leading to the proteolytic cleavage and activation of gasdermin D (GSDMD). Activated GSDMD forms pores in the plasma membrane, causing osmotic cell swelling and membrane rupture, ultimately resulting in pyroptotic cell death.

Key proteins involved in pyroptosis include:

1. **Inflammasomes:** Inflammasomes are multiprotein complexes consisting of a sensor protein (such as NLRP3, NLRC4, AIM2) and an adaptor protein (ASC) that recruits and activates pro-caspase-1. In response to microbial pathogens or cellular stress, inflammasomes assemble and promote the activation of caspase-1.

2. **Caspase-1:** Caspase-1 is a cysteine protease that is activated upon recruitment to inflammasomes. Activated caspase-1 cleaves and activates pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18), which are subsequently released from the cell to trigger inflammation and recruit immune cells.

3. **Gasdermin D (GSDMD):** GSDMD is a pore-forming protein that is cleaved and activated by caspase-1. Activated GSDMD forms pores in the plasma membrane, leading to osmotic cell swelling and membrane rupture, ultimately resulting in pyroptotic cell death.

Pyroptosis serves as an important defense mechanism against intracellular pathogens, including bacteria and viruses, by promoting the rapid elimination of infected cells and triggering inflammatory responses to coordinate the immune defense against microbial infections. However, dysregulated pyroptosis has been implicated in various inflammatory diseases, autoimmune disorders, and tissue damage associated with infection or injury. Therefore, understanding the molecular mechanisms underlying pyroptosis and the roles of key proteins involved in this process is essential for elucidating the pathogenesis of inflammatory diseases and for developing therapeutic strategies to target pyroptosis for the treatment of these disorders.