beta Actin Antibody | Affinity Biosciences LTD|亲科生物官网

$Fig. 3 β-arr2 depletion enhances TLR4-mediated signaling in B lymphocytes of mice with CAIA. a The relative expression of β-arr2 in splenic B lymphocytes from WT control mice and WT mice with CAIA. The intensity of the Western blot bands was analyzed by ImageJ software. b Membrane TLR4 expression on B lymphocytes was detected by flow cytometry, and cells only labeled with secondary antibody were used as negative controls. c Cytoplasmic TLR4 expression was determined using cytosolic proteins extracted from B lymphocytes by ultracentrifugation, and ATP1A1 was used to evaluate the purity of the isolated cytosolic fractions. d The interaction between β-arr2 and TLR4 was assessed by immunofluorescence, and the colocalization rate (%) was analyzed using the built-in software of the confocal microscope. e The mean fluorescence intensity of NF-κB p65 in the nucleus was measured as an indicator of the activity of NF-κB. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 5).$

Fig.

Figure 8.

Figure 10.

Fig.

Figure 6.

Fig.

Figure 5 |The effects of HMGB1 on RCC-stimulated VEGF and VEGFR2 expressions in HUVEC.

Fig.

Figure 5.

Figure 2.

$Figure 5.| Analysis of signaling pathways. (A) Protein levels of NF‑κB P65, p‑P65, IKKβ and IκBα in the total cell fraction of control and co‑cultured Jurkat cells and (B) densitometric analysis of the western blot data.$

Figure 5.

FIGURE 4| Overexpression of uc.

FIGURE 4 | PA treatment attenuated HFD-induced ER stress in rats.

Figure 2| Western blot analysis of PHD2 and HIF-1α protein expression in normal endometrium, atypical endometrial hyperplasia, and endometrial cancer.

FIGURE 3 | SB induces G2/M cell cycle arrest in cervical cancer cells.

Figure 4:| The protein expression of NLRP3, IL-1β, IL-1β17p, and MMP-13 in the control group (A), saline group (B), and pranoprofen group (C) by performing western blot.

Fig.

FIGURE 6 |Uric acid induces SREBP-1c activation and JAK2/STAT3 pathway inhibition in vitro.

$Fig. 3|β-arr2 depletion enhances TLR4-mediated signaling in B lymphocytes of mice with CAIA. c Cytoplasmic TLR4 expression was determined using cytosolic proteins extracted from B lymphocytes by ultracentrifugation, and ATP1A1 was used to evaluate the purity of the isolated cytosolic fractions.$

Fig.

FIGURE 4 |Effect of CT on DOX‐induced hepatocyte apoptosis in rats.

Figure 5.

FIGURE 4|FKN regulated polarization in LPS-induced J774A.

Figure 6 | Knock down of lncRNA ELFN1-AS1 inhibited EMT.

Fig.

Figure 7.

Fig.

Figure 8| The levels of AMPK/CDX2 signaling pathway proteins in the mice colon.

Figure 5.

Figure 8:| Experimental verification reveals FOXP3 involved in TMZ resistance (a).

Figure 4.

Fig.

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells.

Fig.

Figure 3|: Effect of miR-128 knockdown on cardiomyocytes apoptosis induced by Ang II.

Fig.

Figure 3| Protein expression ratio of P-gp and BCRP in the intestines (A) and liver (B).

Fig.

Figure 6 |The effect of sevoflurane on the protein expression of p53 and Nrf2 in liver tissue induced by IR.

FIGURE 2 | Knockdown of FGF9 in the liver of DIO mice exacerbated fatty liver phenotype.

Figure 5 Effects of PAL and Col (1 µM) on NF-κB/NLRP3 and Nrf2 signaling pathways in LPS plus MSU-activated THP-1 cells.

Fig.

Figure 3.

FIGURE 2 A, B, The expression of ALB was lower in hepatocellular carcinoma (HCC) cell lines compared with the normal L02 cells assessed by western blot.

Fig.

Figure 2 Pinin induces EMT and malignant progression in HCC.

Figure 1 Therapeutic effect of HQT on barrier function injury of DSS-induced ulcerative colitis (UC) mice.

Fig.

Figure 4 Effect of PSCP on Nrf2-mediated antioxidant response in HFD-fed mice.

Figure 1.

Figure 6 CD4+ T cells were assessed for the expression of mTOR pathway-associated proteins, ERS-associated proteins, and apoptosis-associated proteins in WT, LCK-mTOR, LCK-TSC1, CLP, CLP+CIRP-Ab, CLP+NAC, LCK-TSC1+CLP, and Lck-mTOR+CLP mouse groups.

Figure 6 Monomeric derivative of paeoniflorin (MDP) inhibits the expression of hypoxia-inducible factor (HIF)-1α through targeted inhibition of GRK2 S670 phosphorylation.

Fig.

Figure 1 FBP1 is reduced in RCC and related to poor survival outcome.

Fig.

Figure 6 RAC induced the apoptosis of the tumor tissue in RCC mice.

FIGURE 6 TS treatment promoted autophagy and clear p-tau.

Figure 3Effect of both concentrations of glutamine on ADAMTS-5, aggrecan, MMP-1, and MMP-13 in chondrocytes treated with IL-1β.

Figure 5 Effect of LBAG on the expression of autophagy and PI3K/AKT pathway-related proteins in rats.

Figure 6 Difference in the expression levels of Akt proteins: A: normal control group, B: normal administration group, C: diabetes control group, D: early administration group, and E: late administration group.

Fig.

Figure 4 Anlotinib inhibits the migration of HSC-3 cells by suppressing the activation of the Akt pathway.

Fig.

Figure 6 Western Blot Validation of Proteins in Neutrophil Activation Networks.

Figure 5 Analysis of the transcriptome and protein expressions in TSPCs treated with different concentrations of UA.

Fig.

FIGURE 5 Licoricidin repressed proliferation and induced apoptosis of MGC-803 cells in vivo.

Figure 7 XFZYD inhibited the IKBα/NF-κB signaling in rat models with CPB-induced ALI.

Figure 7 BA improved the expressions of MYD88, p–NF–κBp65/NF-κBp65, p-IκBa/IκBa, TLR2, and TLR4 in rat lung tissue.

Figure 4 The effects of SD treatment on the levels of the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways' related genes and proteins in the nasal mucosa of AR mice ((n) = 6).

产品:	beta Actin 抗体
货号:	AF7018
描述:	Rabbit polyclonal antibody to beta Actin
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat, Pig, Zebrafish, Bovine, Rabbit, Dog, Monkey, Fish
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	43kDa; 42kD(Calculated).
蛋白号:	P60709
RRID:	AB_2839420

浏览相似产品>>

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 900	现货
100ul	RMB¥ 1500	现货
200ul	RMB¥ 2000	现货
1ml	RMB¥ 5500	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:3000-1:20000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Pig,Zebrafish,Bovine,Rabbit,Dog,Monkey,Fish

预测:

Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

Beta actin antibody detects endogenous levels of total Beta actin.

RRID:

AB_2839420
引用格式: Affinity Biosciences Cat# AF7018, RRID:AB_2839420.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ACTB; Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; β actin;b actin; Actin beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;

抗原和靶标

免疫原:

A synthesized peptide derived from human Beta actin.

Uniprot:

P60709(ACTB_HUMAN) >>Visit HPA database.

基因/基因ID:

ACTB(60)

描述:

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

序列:

P60709 ACTB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P60709 作为底物

P60709

Site	PTM Type	Source
M1	Acetylation	Uniprot
D2	Acetylation	Uniprot
S14	Phosphorylation	Uniprot
C17	S-Nitrosylation	Uniprot
K18	Methylation	Uniprot
K18	Ubiquitination	Uniprot
S33	Phosphorylation	Uniprot
K50	Acetylation	Uniprot
K50	Methylation	Uniprot
K50	Ubiquitination	Uniprot
S52	Phosphorylation	Uniprot
Y53	Phosphorylation	Uniprot
S60	Phosphorylation	Uniprot
K61	Acetylation	Uniprot
K61	Sumoylation	Uniprot
K61	Ubiquitination	Uniprot
T66	Phosphorylation	Uniprot
K68	Methylation	Uniprot
K68	Sumoylation	Uniprot
Y69	Phosphorylation	Uniprot
H73	Methylation	Uniprot
T77	Phosphorylation	Uniprot
K84	Methylation	Uniprot
K84	Sumoylation	Uniprot
K84	Ubiquitination	Uniprot
T89	Phosphorylation	Uniprot
Y91	Phosphorylation	Uniprot
T106	Phosphorylation	Uniprot
K113	Acetylation	Uniprot
K113	Sumoylation	Uniprot
K113	Ubiquitination	Uniprot
T120	Phosphorylation	Uniprot
Y143	Phosphorylation	Uniprot
S155	Phosphorylation	Uniprot
T160	Phosphorylation	Uniprot
T162	Phosphorylation	Uniprot
Y166	Phosphorylation	Uniprot
Y169	Phosphorylation	Uniprot
T186	Phosphorylation	Uniprot
Y188	Phosphorylation	Uniprot
K191	Acetylation	Uniprot
K191	Methylation	Uniprot
K191	Ubiquitination	Uniprot
T194	Phosphorylation	Uniprot
Y198	Phosphorylation	Uniprot
S199	Phosphorylation	Uniprot
T201	Phosphorylation	Uniprot
T202	Phosphorylation	Uniprot
T203	Phosphorylation	Uniprot
K213	Acetylation	Uniprot
K213	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
C217	S-Nitrosylation	Uniprot
Y218	Phosphorylation	Uniprot
T229	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
S235	Phosphorylation	Uniprot
K238	Ubiquitination	Uniprot
S239	Phosphorylation	Uniprot
Y240	Phosphorylation	Uniprot
T249	Phosphorylation	Uniprot
C257	S-Nitrosylation	Uniprot
S265	Phosphorylation	Uniprot
S271	Phosphorylation	Uniprot
C272	S-Nitrosylation	Uniprot
K284	Sumoylation	Uniprot
K284	Ubiquitination	Uniprot
C285	S-Nitrosylation	Uniprot
K291	Sumoylation	Uniprot
K291	Ubiquitination	Uniprot
Y294	Phosphorylation	Uniprot
T297	Phosphorylation	Uniprot
S300	Phosphorylation	Uniprot
T303	Phosphorylation	Uniprot
T304	Phosphorylation	Uniprot
Y306	Phosphorylation	Uniprot
K315	Acetylation	Uniprot
K315	Sumoylation	Uniprot
K315	Ubiquitination	Uniprot
T318	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot
T324	Phosphorylation	Uniprot
K326	Acetylation	Uniprot
K326	Methylation	Uniprot
K326	Sumoylation	Uniprot
K326	Ubiquitination	Uniprot
K328	Acetylation	Uniprot
K328	Sumoylation	Uniprot
K328	Ubiquitination	Uniprot
K336	Sumoylation	Uniprot
S348	Phosphorylation	Uniprot
K359	Ubiquitination	Uniprot
Y362	Phosphorylation	Uniprot
S365	Phosphorylation	Uniprot
S368	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot
C374	S-Nitrosylation	Uniprot

研究背景

P60709_ACTB_HUMAN

功能:

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

翻译修饰:

ISGylated.

Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.

Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).

N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

细胞定位:

Cytoplasm>Cytoskeleton. Nucleus.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

亚基结构:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21 (By similarity). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes. Interacts with FAM107A.

蛋白家族:

Belongs to the actin family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer. Molecular Cancer (PubMed: 30744636) [IF=37.3]

Application: WB Species: mouse Sample: cells

Fig. 4| circKIF4A acts as a ceRNA to regulate KIF4A a The predicted binding sites of miR-375 within the KIF4A 3’UTR. b Cells were transfected and luciferase assay was performed. c Cells were transfected and KIF4A expression was detected by qRT-PCR. d The KIF4A expression was detected by western blot (left) and quantified (right).

2). Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis. Cell stem cell (PubMed: 38232727) [IF=23.9]

3). A Checkpoint-Regulatable Immune Niche Created by Injectable Hydrogel for Tumor Therapy. ADVANCED FUNCTIONAL MATERIALS [IF=19.0]

4). In situ MUC1-specific CAR engineering of tumor-supportive macrophages stimulates tumoricidal immunity against pancreatic adenocarcinoma. Nano Today [IF=17.4]

5). Mitochondrial Disruption Nanosystem Simultaneously Depressed Programmed Death Ligand-1 and Transforming Growth Factor-β to Overcome Photodynamic Immunotherapy Resistance. ACS nano (PubMed: 38227812) [IF=17.1]

6). Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma. Cancer communications (London, England) (PubMed: 37920878) [IF=16.2]

7). Polydopamine nanoparticles as dual-task platform for osteoarthritis therapy: A scavenger for reactive oxygen species and regulator for cellular powerhouses. Chemical Engineering Journal [IF=15.1]

8). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH (PubMed: 32710621) [IF=14.9]

Application: WB Species: mouse Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.

9). Emodin attenuates severe acute pancreatitis-associated acute lung injury by suppressing pancreatic exosome-mediated alveolar macrophage activation. Acta Pharmaceutica Sinica B (PubMed: 36213542) [IF=14.5]

10). LncRNA DACH1 protects against pulmonary fibrosis by binding to SRSF1 to suppress CTNNB1 accumulation. Acta Pharmaceutica Sinica B (PubMed: 36176913) [IF=14.5]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.

beta Actin Antibody - #AF7018

产品推荐

相关下载

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

抗原和靶标

种属预测

翻译修饰 - P60709 作为底物

研究背景

研究领域

文献引用

限制条款