WB, IF/ICC, IHC, ELISA

Hm, Ms, Rt, Mk, Rb

货号	产品	规格	来源	应用	反应	文献引用
AF6237	CDK2 Ab	20ul	Rabbit	WB,IF/ICC	Hm,Ms,Rt	10
AF6324	p27 Kip1 Ab	20ul	Rabbit	WB,IF/ICC	Hm,Ms,Rt	14
DF6386	Cyclin D1 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	7
DF6448	CDK6 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	7
DF6102	CDK4 Ab	20ul	Rabbit	WB,IHC	Hm,Ms,Rt	22
AF6251	Cyclin D3 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Mk	2
AF0620	p18 INK4c Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Mk	2
AF6290	p21 Cip1 Ab	20ul	Rabbit	WB,IF/ICC	Hm,Ms,Rt	47
AF7011	Tubulin beta Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	98
S0001	Goat Anti-Rabbit IgG HRP	100ul	Goat	WB,IHC,ELISA	Rb	506

The cell cycle regulation pathway governs the orderly progression of cells through distinct phases of the cell cycle, including interphase (G1, S, and G2 phases) and mitosis (M phase). Proper regulation of the cell cycle is essential for maintaining genome integrity, ensuring accurate duplication and segregation of chromosomes, and controlling cell proliferation.

Store at -20 °C. Stable for 12 months from date of receipt.

The cell cycle regulation pathway governs the orderly progression of cells through distinct phases of the cell cycle, including interphase (G1, S, and G2 phases) and mitosis (M phase). Proper regulation of the cell cycle is essential for maintaining genome integrity, ensuring accurate duplication and segregation of chromosomes, and controlling cell proliferation.

Key events and proteins involved in cell cycle regulation include:

1. **Cyclins and Cyclin-Dependent Kinases (CDKs):** Cyclins are regulatory proteins that undergo periodic fluctuations in expression levels throughout the cell cycle. They bind to and activate CDKs, which are serine/threonine kinases that phosphorylate target proteins to drive cell cycle progression. Different cyclin-CDK complexes regulate specific transitions between cell cycle phases. For example, cyclin D-CDK4/6 complexes promote entry into the cell cycle from the G1 phase, cyclin E-CDK2 complexes drive progression through the G1/S transition, and cyclin A-CDK2 and cyclin B-CDK1 complexes regulate DNA replication and entry into mitosis, respectively.

2. **Cyclin-Dependent Kinase Inhibitors (CKIs):** CKIs are negative regulators of CDK activity that bind to and inhibit cyclin-CDK complexes, thereby blocking cell cycle progression. CKIs can be classified into two families: the INK4 family (including p16INK4a, p15INK4b, p18INK4c, and p19INK4d) that specifically inhibits CDK4/6 activity, and the Cip/Kip family (including p21Cip1, p27Kip1, and p57Kip2) that inhibits multiple cyclin-CDK complexes.

3. **Retinoblastoma Protein (Rb):** Rb is a tumor suppressor protein that plays a key role in regulating the G1/S transition. In its hypophosphorylated form, Rb binds to and inhibits the transcription factor E2F, thereby suppressing the expression of genes required for DNA replication. Phosphorylation of Rb by cyclin D-CDK4/6 and cyclin E-CDK2 complexes releases its inhibition of E2F, allowing progression into the S phase.

4. **Anaphase-Promoting Complex/Cyclosome (APC/C):** APC/C is a multisubunit ubiquitin ligase complex that regulates the metaphase-to-anaphase transition and exit from mitosis. APC/C targets several key proteins for ubiquitination and degradation, including securin and cyclin B, leading to activation of separase and degradation of cyclin B, respectively, which are necessary for sister chromatid separation and mitotic exit.

5. **Checkpoint Proteins:** Checkpoint proteins monitor the fidelity of DNA replication and chromosome segregation and activate cell cycle checkpoints in response to DNA damage or other abnormalities. These include proteins such as p53, ATM, ATR, CHK1, and CHK2, which coordinate DNA repair, arrest cell cycle progression, or induce apoptosis if DNA damage is irreparable.

Proper regulation of the cell cycle is essential for normal development, tissue homeostasis, and prevention of diseases such as cancer. Dysregulation of cell cycle proteins can lead to uncontrolled cell proliferation, genomic instability, and tumorigenesis. Therefore, understanding the molecular mechanisms governing cell cycle regulation and the roles of key proteins involved in this process is crucial for elucidating the pathogenesis of diseases and developing targeted therapies for cancer and other disorders.