FRA1 Antibody - #DF3096

Fig. 5. The correlation of ETV4 and FOSL1 in cell lines and other tumor type tissues. (A). The clinical distribution of ETV4 and FOSL1 were analyzed by using the TCGA ccRCC data set. (B). The protein level were determined by western blotting showing that ETV4 and FOSL1 were correlatively expressed in ccRCC cell lines. (C), (E) and (G). Transient suppression of ETV4 in ACHN, ETV4 and FOSL1 mRNA level was detected by qPCR. Data represent the mean ± S.D. ***p < 0.0001. ETV4 and FOSL1 expression were measured by immunoblotting. (D), (F) and (H). Transient suppression of ETV4 in SN12C, ETV4 and FOSL1 mRNA level was detected by qPCR. Data represent the mean ± S.D. ***p < 0.0001. ETV4 and FOSL1 expression were measured by immunoblotting. (I) Localization of Vimentin in ACHN NC, ACHN si# 1 cells by confocal immunofluorescence analysis. (J). Suppression of FOSL1 dramatically reduced the migration ability of the cells as determined by a wound-healing assay. Yellow dashed lines denote the margins of the wound. (K). The quantified data of wound width of transwell. Columns, average of three independent experiments; bars, SD. *p < 0.05, Student t-test. (L) and (M). The migratory ability of overexpressed ETV4 and suppressed FOSL1 in ACHN cells. Data represent the mean ± S.D. *p < 0.05. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)