产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3179, RRID:AB_2835402.
展开/折叠
Beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; GTK, mouse, homolog of; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; PTK5 protein tyrosine kinase 5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK;
抗原和靶标
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
- P42685 FRK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQVLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLKIQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDYFETDSSYSDANNFIR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P42685 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| Y193 | Phosphorylation | Uniprot | |
| Y221 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| Y317 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | P42685 (FRK) | Uniprot |
| S389 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K409 | Acetylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| Y497 | Phosphorylation | P41240 (CSK) | Uniprot |
| S498 | Phosphorylation | Uniprot |
翻译修饰 - P42685 作为激酶
研究背景
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Cytoplasm. Nucleus.
Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery.
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
Interacts (via the SH3-domain) with PTEN. Interacts with RB1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
限制条款
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