GRK6 Antibody - #DF3180

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产品: GRK6 抗体
货号: DF3180
描述: Rabbit polyclonal antibody to GRK6
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 65 KD; 66kD(Calculated).
蛋白号: P43250
RRID: AB_2835403

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
GRK6 Antibody detects endogenous levels of total GRK6.
RRID:
AB_2835403
引用格式: Affinity Biosciences Cat# DF3180, RRID:AB_2835403.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ32135; G protein coupled receptor kinase 6; G protein coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; Gprk6; Grk6; GRK6_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P43250(GRK6_HUMAN) >>Visit HPA database.

基因/基因ID:
GRK6(2870)
表达:
P43250 GRK6_HUMAN:

Widely expressed.

序列:
MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCSDSEEELPTRL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P43250 作为底物

Site PTM Type Enzyme
T10 Phosphorylation
K14 Ubiquitination
T106 Phosphorylation
S111 Phosphorylation
T113 Phosphorylation
K139 Ubiquitination
K183 Ubiquitination
K194 Ubiquitination
K210 Ubiquitination
K226 Ubiquitination
K235 Acetylation
K235 Ubiquitination
K240 Acetylation
K240 Ubiquitination
K313 Ubiquitination
K343 Ubiquitination
K358 Ubiquitination
K402 Ubiquitination
K426 Ubiquitination
K443 Ubiquitination
K466 Ubiquitination
S484 Phosphorylation P43250 (GRK6)
T485 Phosphorylation P43250 (GRK6)
K487 Ubiquitination
S557 Phosphorylation
S566 Phosphorylation
S568 Phosphorylation

翻译修饰 - P43250 作为激酶

Substrate Site Source
O14745 (SLC9A3R1) S290 Uniprot
O75581 (LRP6) S1448 Uniprot
O75581 (LRP6) S1490 Uniprot
O75581 (LRP6) S1508 Uniprot
O75581 (LRP6) T1572 Uniprot
O75581 (LRP6) S1590 Uniprot
P07550 (ADRB2) S355 Uniprot
P07550 (ADRB2) S356 Uniprot
P08069 (IGF1R) S1321 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P30411-2 (BDKRB2) S346 Uniprot
P30411-2 (BDKRB2) S348 Uniprot
P30411 (BDKRB2) S366 Uniprot
P30411 (BDKRB2) S373 Uniprot
P30411-1 (BDKRB2) S375 Uniprot
P37840-1 (SNCA) S9 Uniprot
P37840 (SNCA) S129 Uniprot
P43250 (GRK6) S484 Uniprot
P43250-3 (GRK6) T485 Uniprot
P61073 (CXCR4) S324 Uniprot
P61073 (CXCR4) S325 Uniprot
P61073 (CXCR4) S330 Uniprot
P61073 (CXCR4) S339 Uniprot
Q15722 (LTB4R) T308 Uniprot

研究背景

功能:

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

翻译修饰:

It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.

细胞定位:

Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

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