产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3188, RRID:AB_2835411.
展开/折叠
Casein kinase 1 epsilon; Casein kinase I; Casein kinase I epsilon; Casein kinase I isoform epsilon; CKI epsilon; CKI-epsilon; CKIe; CKIepsilon; CSNK 1E; CSNK1E; DBT; Doubletime, Drosophila, homolog of; EC 2.7.11.1; epsilon isoform; HCK IE; KC1E; KC1E_HUMAN; kinase CK1 epsilon; MGC 10398;
抗原和靶标
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
- P49674 KC1E_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49674 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| K45 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| S323 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T325 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T334 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T337 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| R342 | Methylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S377 | Phosphorylation | Uniprot | |
| R382 | Methylation | Uniprot | |
| S389 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S390 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T407 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S408 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
翻译修饰 - P49674 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O14640 (DVL1) | S139 | Uniprot |
| O14640 (DVL1) | S142 | Uniprot |
| O14641 (DVL2) | S143 | Uniprot |
| O14641 (DVL2) | T224 | Uniprot |
| O15055-1 (PER2) | S662 | Uniprot |
| O15055-1 (PER2) | S665 | Uniprot |
| O15055 (PER2) | S668 | Uniprot |
| O15055-1 (PER2) | S671 | Uniprot |
| O15055-1 (PER2) | S674 | Uniprot |
| O60716 (CTNND1) | S268 | Uniprot |
| O75581 (LRP6) | S1420 | Uniprot |
| O75581 (LRP6) | S1430 | Uniprot |
| O75581 (LRP6) | S1431 | Uniprot |
| O95863 (SNAI1) | S104 | Uniprot |
| O95863 (SNAI1) | S107 | Uniprot |
| P12830 (CDH1) | S844 | Uniprot |
| P25054 (APC) | S1279 | Uniprot |
| P25054 (APC) | S1392 | Uniprot |
| P25054-1 (APC) | S1504 | Uniprot |
| P25054-1 (APC) | S1505 | Uniprot |
| P25054-1 (APC) | S1507 | Uniprot |
| P25054-1 (APC) | S1510 | Uniprot |
| P35222 (CTNNB1) | S45 | Uniprot |
| P46937 (YAP1) | S400 | Uniprot |
| P46937 (YAP1) | S403 | Uniprot |
| P49674 (CSNK1E) | T44 | Uniprot |
| P49674 (CSNK1E) | S323 | Uniprot |
| P49674 (CSNK1E) | T325 | Uniprot |
| P49674 (CSNK1E) | T334 | Uniprot |
| P49674 (CSNK1E) | T337 | Uniprot |
| P49674 (CSNK1E) | S368 | Uniprot |
| P49674 (CSNK1E) | S405 | Uniprot |
| P49674 (CSNK1E) | T407 | Uniprot |
| P49674 (CSNK1E) | S408 | Uniprot |
| P55957 (BID) | T59 | Uniprot |
| P55957 (BID) | S64 | Uniprot |
| P56645-1 (PER3) | S622 | Uniprot |
| P56645-1 (PER3) | S625 | Uniprot |
| P56645-1 (PER3) | S628 | Uniprot |
| P56645-1 (PER3) | S633 | Uniprot |
| P56645-1 (PER3) | S634 | Uniprot |
| P56645-1 (PER3) | T635 | Uniprot |
| Q13541 (EIF4EBP1) | T41 | Uniprot |
| Q13541 (EIF4EBP1) | T50 | Uniprot |
| Q8TD43 (TRPM4) | S839 | Uniprot |
| Q9BZL6 (PRKD2) | S244 | Uniprot |
| Q9GZV5 (WWTR1) | S314 | Uniprot |
| Q9Y6Q9 (NCOA3) | S102 | Uniprot |
研究背景
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.
Cytoplasm. Nucleus.
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity). Interacts with PER1. Interacts with ANKRD6 (By similarity). Interacts with DBNDD2. Interacts with LRP5 and LRP6. Interacts with SOCS3. Interacts with SNAI1 (via zinc fingers). Interacts with DDX3X; the interaction greatly improves CSNK1E affinity for ATP and enhances DVL2 phosphorylation (By similarity).
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
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