产品: RANKL 抗体
货号: AF0313
来源: Rabbit
应用: WB, IHC, IF/ICC, ELISA(peptide)
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 40kD; 35kD(Calculated).
蛋白号: O14788
RRID: AB_2833477

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 50ul ¥1250 现货
 100ul ¥2300 现货
 200ul ¥3000 现货

货期: 当天发货

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%)
克隆:
Polyclonal
特异性:
RANKL Antibody detects endogenous levels of total RANKL.
RRID:
AB_2833477
引用格式: Affinity Biosciences Cat# AF0313, RRID:AB_2833477.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF related activation induced cytokine; TNF-related activation-induced cytokine; TNF11_HUMAN; TNFSF 11; Tnfsf11; TRANCE; Tumor necrosis factor (ligand) superfamily member 11; Tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, soluble form;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达特异性:
O14788 TNF11_HUMAN:

Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

蛋白描述:
TNFSF11 Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
蛋白序列:
MRRASRDYTKYLRGSEEMGGGPGAPHEGPLHAPPPPAPHQPPAASRSMFVALLGLGLGQVVCSVALFFYFRAQMDPNRISEDGTHCIYRILRLHENADFQDTTLESQDTKLIPDSCRRIKQAFQGAVQKELQHIVGSQHIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
90
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O14788 作为底物

Site PTM Type Enzyme
S137 Phosphorylation

研究背景

功能:

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

翻译修饰:

The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

细胞定位:

Cell membrane>Single-pass type II membrane protein.

Cell membrane>Single-pass type II membrane protein.

Cytoplasm.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

亚基结构:

Homotrimer (By similarity). Interacts with TNFRSF11B. Interacts with TNFRSF11A. Interacts with FBN1 (via N-terminal domain) in a Ca(+2)-dependent manner (By similarity).

蛋白家族:

Belongs to the tumor necrosis factor family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

文献引用

1). Ye C et al. IGFBP7 acts as a negative regulator of RANKL-induced osteoclastogenesis and oestrogen deficiency-induced bone loss. Cell Prolif 2020 Feb;53(2):e12752 (PubMed: 31889368) [IF=8.755]

Application: IF/ICC    Species: mice    Sample: RAW264.7 cells

FIGURE 1 IGFBP7 suppressed RANKL-induced osteoclastogenesis in vitro. A-B, CCK-8 analysis was conducted to evaluate cell viability after treated by different concentration of recombinant IGFBP7 protein for 48 and 96 h. C, BMMs were cultured with different concentration of recombinant IGFBP7 protein in osteoclastogenic medium for 5 d, and TRAP staining was performed. Scale bar = 200 µm. D-E, The number and area of osteoclasts were calculated. F-H, BMMs were treated with either vehicle or 1000 ng/mL recombinant IGFBP7 protein from days 1-3 (early stage), days 3-5 (late stage) or days 1-5 (early + late stage) during osteoclastogenesis. The number and area of osteoclasts were calculated. Scale bar = 200 µm. I and K, Representative fluorescence images showed that recombinant IGFBP7 treatment significantly decreased the size of F-actin ring structures. Scale bar = 100 µm. J and L, Representative SEM (scanning electron microscopy) images showed that recombinant IGFBP7 treatment decreased the area of bone resorption pits. Scale bars = 200 µm. *P < .05, **P < .01 vs the control group

Application: IF/ICC    Species: mouse    Sample: osteoblastic cel

FIGURE 1 |IGFBP7 suppressed RANKL-induced osteoclastogenesis in vitro.I and K, Representative fluorescence images showed that recombinant IGFBP7 treatment significantly decreased the size of F-actin ring structures. Scale bar = 100 µm. J and L, Representative SEM (scanning electron microscopy) images showed that recombinant IGFBP7 treatment decreased the area of bone resorption pits. Scale bars = 200 µm. *P < .05, **P < .01 vs the control group

Application: WB    Species: mouse    Sample: MC3T3-E1

Figure S1.| The results of western-blot analysis showed that different concentrations of recombinant IGFBP7 increased the protein expression of OPG in osteoblastic cell line MC3T3-E1, whereas the expression of RANKL was not affected after 3 days’ IGFBP7 treatment. OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor-κB ligand.

2). Chen X et al. Nirogacestat suppresses RANKL-Induced osteoclast formation in vitro and attenuates LPS-Induced bone resorption in vivo. Exp Cell Res 2019 Jun 15 (PubMed: 31211955) [IF=4.145]

Application: IHC    Species: mouse    Sample: BMMs

Fig. 7.| Histological analysis of the inhibitory effect of PF on LPS-induced bone resorption. (A–C) Representative images of HE and TRAP staining, showing the reduced osteolytic legion and TRAP-positive OCs in the PF-treated groups. (D–G) Representative images of IHC staining of RANKL, OPG, OCN and TNF-α. (H) TRAPpositive OCs number. (H) Quantitative analysis of the expression of RANKL, OPG and RANKL/OPG ratio. The data are presented as the mean ± SD (*p < 0.05,**p < 0.01, ***p < 0.001).

3). Liu D et al. Role of microRNA-19b-3p on osteoporosis after experimental spinal cord injury in rats. Arch Biochem Biophys 2021 Feb 12;108805. (PubMed: 33587904) [IF=4.114]

Application: WB    Species: Rat    Sample: osteoblasts

Figure 2. MiR-19b-3p inhibits osteoblast differentiation. (A) RT-PCR analysis was used to detect miRNA-19b-3p expression levels in BMSC-derived osteoblasts after miR-19b-3p-overexpressed or knockdown by corresponding lentivirus. (B) ALP activity in BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by corresponding lentivirus. (C) RT-PCR was used to detect EBF2 mRNA expression levels in Journal Pre-proof 20 BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by corresponding lentivirus. Western blot assay was used to detect (D and E) EBF2, (F-H) RANKL and OPG protein expression levels in BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by corresponding lentivirus. (I) The ratio of OPG to RANKL in BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by corresponding lentivirus (J) Alizarin red staining was used to detect cell mineralization at day 21 after differentiation after miRNA-19b-3p overexpression or knockdown at 100×. Data are analyzed using unpaired Student’s t-tests and presented as the mean ± SD from three independent experiments. * p < 0.05; ** p < 0.01 vs. the indicated group.

4). Lin H et al. The role of gut microbiota metabolite trimethylamine N-oxide in functional impairment of bone marrow mesenchymal stem cells in osteoporosis disease. Ann Transl Med 2020 Aug;8(16):1009. (PubMed: 32953809) [IF=3.616]

Application: WB    Species: Human    Sample: bone marrow mesenchymal stem cells (BMSCs)

Figure 4 TMAO promotes the adipogenic differentiation and inhibits the osteogenic differentiation of BMSCs. (A) Adipogenic differentiation was detected by oil red O staining; (B) osteogenic differentiation was detected by alizarin red staining; (C) the expressions of NF-κB, PPARγ, C/EBP-α, Runx2 and OPN in BMSCs were measured by WB. Actin was used as an internal control; (D) quantification of NF-κB/Actin, PPARγ/Actin, C/EBP-α/Actin, Runx2/Actin, and OPN/Actin protein expression. *, P<0.05, **, P<0.01, ***, P<0.001 vs. OIM or AIM group. TMAO, trimethylamine N-oxide; BMSCs, bone marrow mesenchymal stem cells. NF-κB, nuclear factor-κB; PPARγ, Peroxisome proliferator-activated receptor gamma; C/EBP-α, CCAAT/enhancer-binding protein alpha; Runx2, runt-related transcription factor 2; OPN, Osteopontin.

5). He M et al. Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption. Arch Oral Biol 2021 Jun;126:105117. (PubMed: 33845260) [IF=2.640]

Application: WB    Species: Rat    Sample: IF cells and MF cells

Fig. 5. Differential expression patterns of tooth eruption-related genes and proteins between IF cells and MF cells. (A) Non-induced IF cells and MF cells showed different gene expression patterns; Data are mean ± SD of n = 3 replicates, one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. (B) Non-induced and induced IF cells and MF cells showed different protein expression patterns. After induction with CLCCM and HERSCM, there were no significant changes of the expression patterns of tooth eruption-related proteins in IF cells and MF cells. (C) The grey value ratios of Western blotting results; Data are mean ± SD of n = 3 replicates, one-way ANOVA followed by Tukey post hoc test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. (IF1, MF1 represent the cells cultured by α-MEM; IF2, MF2 represent the cells cultured by α-MEM + CLCCM; IF3, MF3 represent the cells cultured by α-MEM+HERSCM).

6). He M et al. Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption. Arch Oral Biol 2021 Jun;126:105117. (PubMed: 33845260) [IF=2.640]

Application: WB    Species: Rat    Sample: Incisor dental follicle (IF) cells and molar dental follicle (MF) cells

Fig. 5. Differential expression patterns of tooth eruption-related genes and proteins between IF cells and MF cells. (A) Non-induced IF cells and MF cells showed different gene expression patterns; Data are mean ± SD of n = 3 replicates, one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. (B) Non-induced and induced IF cells and MF cells showed different protein expression patterns. After induction with CLCCM and HERSCM, there were no significant changes of the expression patterns of tooth eruption-related proteins in IF cells and MF cells. (C) The grey value ratios of Western blotting results; Data are mean ± SD of n = 3 replicates, one-way ANOVA followed by Tukey post hoc test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. (IF1, MF1 represent the cells cultured by α-MEM; IF2, MF2 represent the cells cultured by α-MEM + CLCCM; IF3, MF3 represent the cells cultured by α-MEM+HERSCM).

7). The expressions of tooth eruption relevant genes are different in incisors and molars dental follicle cells in rat: an in vitro study.

8). Effects of low frequency pulsed electromagnetic fields exposure on the condylar subchondral bone at the early stage of temporomandibular joint osteoarthritis.

9). Low frequency pulsed electromagnetic fields exposure alleviate the abnormal subchondral bone remodeling at the early stage of temporomandibular joint osteoarthritis.

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