(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3056, RRID:AB_2835439.
展开/折叠
Hbo 1; HBO1; HBOa; Histone acetyltransferase binding to hORC1; Histone acetyltransferase binding to ORC1; Histone acetyltransferase KAT7; Histone acetyltransferase MYST2; K(lysine) acetyltransferase 7; KAT 7; KAT7; Lysine acetyltransferase 7; MOZ; MOZ YBF2/SAS3 SAS2 and TIP60 protein; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST 2; MYST histone acetyltransferase 2; MYST protein 2; MYST-2; MYST2; MYST2_HUMAN; SAS 2; SAS2 and TIP60 protein 2; TIP60 protein 2; YBF2/SAS3; ZC2HC7;
抗原和靶标
- O95251 KAT7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTPPKGT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95251 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S64 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T88 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| K90 | Ubiquitination | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K160 | Acetylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| K171 | Acetylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| K184 | Sumoylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K229 | Acetylation | Uniprot | |
| K261 | Acetylation | Uniprot | |
| K269 | Acetylation | Uniprot | |
| K270 | Acetylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| K323 | Acetylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| T331 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| K376 | Methylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| K432 | Acetylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| K596 | Acetylation | Uniprot | |
| T606 | Phosphorylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| T611 | Phosphorylation | Uniprot |
研究背景
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (By similarity). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1. Specifically represses AR-mediated transcription.
Phosphorylation at Ser-57 by PLK1 during mitosis seems important for prereplicative complex formation and DNA replication licensing, and requires prior phosphorylation at Thr-85 and Thr-88 by CDK1. Phosphorylated by MAP2K1, which accelerates its degradation (By similarity).
Autoacetylation at Lys-432 is required for proper function.
Ubiquitinated at Lys-338, leading to proteasomal degradation.
Nucleus>Nucleoplasm. Cytoplasm>Cytosol. Nucleus.
Note: Associates with replication origins specifically during the G1 phase of the cell cycle (PubMed:18832067).
Ubiquitously expressed, with highest levels in testis.
Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with MCM2 and ORC1. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone. Interacts with CDT1. Interacts with MAP2K1 and CUL1 (By similarity).
The C2HC MYST-type zinc finger is required for interaction with MCM2 and ORC1.
The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
Belongs to the MYST (SAS/MOZ) family.
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