产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3072, RRID:AB_2835455.
展开/折叠
CKM; CKMM; Creatine kinase M; Creatine kinase M chain; Creatine kinase M type; Creatine kinase M-type; Creatine kinase muscle; Creatine kinase, muscle type; KCRM_HUMAN; M-CK; MCK; Muscle creatine kinase;
抗原和靶标
- P06732 KCRM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P06732 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y14 | Phosphorylation | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K170 | Acetylation | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| Y174 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| R215 | Methylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K265 | Acetylation | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| S372 | Phosphorylation | Uniprot |
研究背景
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Cytoplasm.
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Belongs to the ATP:guanido phosphotransferase family.
研究领域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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